Comparative analysis of hemocyte phagocytosis between six species of arthropods as measured by flow cytometry

Jonathan D. Oliver; J. Dusty Loy; Grishma Parikh; Lyric Bartholomay

doi:10.1016/j.jip.2011.07.004

Comparative analysis of hemocyte phagocytosis between six species of arthropods as measured by flow cytometry

Jonathan D. Oliver, J. Dusty Loy, Grishma Parikh, Lyric Bartholomay

Entomology

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

Phagocytosis of pathogens by hemocytes is a rapid-acting immune response and represents a primary means of limiting microbial infection in some species of arthropods. To survey the relative capacity of hemocyte phagocytosis as a function of the arthropod immune response, we examined the extent of phagocytosis among a wide taxonomic range of arthropod species including a decapod crustacean (Litopenaeus vannamei), three ixodid tick species (Amblyomma americanum, Dermacentor variabilis, and Ixodes scapularis), a mosquito species (Aedes aegypti), and a larval moth (Manduca sexta). Injected fluorescent beads were used as a model to elicit phagocytosis and were measured by flow cytometry, a technique provided in detail that may be adapted for use with any species of arthropod. The data indicated that smaller arthropods generally had a higher proportion of phagocytic cells than larger arthropods.

Original language	English (US)
Pages (from-to)	126-130
Number of pages	5
Journal	Journal of Invertebrate Pathology
Volume	108
Issue number	2
DOIs	https://doi.org/10.1016/j.jip.2011.07.004
State	Published - Oct 2011

Bibliographical note

Funding Information:
Ticks were obtained from the Oklahoma State University Tick Rearing Facility. Larval M. sexta were provided by the Iowa State University Insect Zoo. This journal paper of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa, Project 5111, is based upon work supported by the Hatch Act and State of Iowa funds. Flow cytometry was performed at the Iowa State University Flow Cytometry Facility by Shawn Rigby.

Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.

Keywords

Flow cytometry
Hemocyte
Immune response
Immunity
Phagocytosis

Access

10.1016/j.jip.2011.07.004

OpenUrl availability

Full text

Cite this

@article{ab817e99dcde42259b1fdb04da976891,

title = "Comparative analysis of hemocyte phagocytosis between six species of arthropods as measured by flow cytometry",

abstract = "Phagocytosis of pathogens by hemocytes is a rapid-acting immune response and represents a primary means of limiting microbial infection in some species of arthropods. To survey the relative capacity of hemocyte phagocytosis as a function of the arthropod immune response, we examined the extent of phagocytosis among a wide taxonomic range of arthropod species including a decapod crustacean (Litopenaeus vannamei), three ixodid tick species (Amblyomma americanum, Dermacentor variabilis, and Ixodes scapularis), a mosquito species (Aedes aegypti), and a larval moth (Manduca sexta). Injected fluorescent beads were used as a model to elicit phagocytosis and were measured by flow cytometry, a technique provided in detail that may be adapted for use with any species of arthropod. The data indicated that smaller arthropods generally had a higher proportion of phagocytic cells than larger arthropods.",

keywords = "Flow cytometry, Hemocyte, Immune response, Immunity, Phagocytosis",

author = "Oliver, {Jonathan D.} and {Dusty Loy}, J. and Grishma Parikh and Lyric Bartholomay",

note = "Funding Information: Ticks were obtained from the Oklahoma State University Tick Rearing Facility. Larval M. sexta were provided by the Iowa State University Insect Zoo. This journal paper of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa, Project 5111, is based upon work supported by the Hatch Act and State of Iowa funds. Flow cytometry was performed at the Iowa State University Flow Cytometry Facility by Shawn Rigby. Copyright: Copyright 2012 Elsevier B.V., All rights reserved.",

year = "2011",

month = oct,

doi = "10.1016/j.jip.2011.07.004",

language = "English (US)",

volume = "108",

pages = "126--130",

journal = "Journal of Invertebrate Pathology",

issn = "0022-2011",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Comparative analysis of hemocyte phagocytosis between six species of arthropods as measured by flow cytometry

AU - Oliver, Jonathan D.

AU - Dusty Loy, J.

AU - Parikh, Grishma

AU - Bartholomay, Lyric

N1 - Funding Information: Ticks were obtained from the Oklahoma State University Tick Rearing Facility. Larval M. sexta were provided by the Iowa State University Insect Zoo. This journal paper of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa, Project 5111, is based upon work supported by the Hatch Act and State of Iowa funds. Flow cytometry was performed at the Iowa State University Flow Cytometry Facility by Shawn Rigby. Copyright: Copyright 2012 Elsevier B.V., All rights reserved.

PY - 2011/10

Y1 - 2011/10

N2 - Phagocytosis of pathogens by hemocytes is a rapid-acting immune response and represents a primary means of limiting microbial infection in some species of arthropods. To survey the relative capacity of hemocyte phagocytosis as a function of the arthropod immune response, we examined the extent of phagocytosis among a wide taxonomic range of arthropod species including a decapod crustacean (Litopenaeus vannamei), three ixodid tick species (Amblyomma americanum, Dermacentor variabilis, and Ixodes scapularis), a mosquito species (Aedes aegypti), and a larval moth (Manduca sexta). Injected fluorescent beads were used as a model to elicit phagocytosis and were measured by flow cytometry, a technique provided in detail that may be adapted for use with any species of arthropod. The data indicated that smaller arthropods generally had a higher proportion of phagocytic cells than larger arthropods.

AB - Phagocytosis of pathogens by hemocytes is a rapid-acting immune response and represents a primary means of limiting microbial infection in some species of arthropods. To survey the relative capacity of hemocyte phagocytosis as a function of the arthropod immune response, we examined the extent of phagocytosis among a wide taxonomic range of arthropod species including a decapod crustacean (Litopenaeus vannamei), three ixodid tick species (Amblyomma americanum, Dermacentor variabilis, and Ixodes scapularis), a mosquito species (Aedes aegypti), and a larval moth (Manduca sexta). Injected fluorescent beads were used as a model to elicit phagocytosis and were measured by flow cytometry, a technique provided in detail that may be adapted for use with any species of arthropod. The data indicated that smaller arthropods generally had a higher proportion of phagocytic cells than larger arthropods.

KW - Flow cytometry

KW - Hemocyte

KW - Immune response

KW - Immunity

KW - Phagocytosis

UR - http://www.scopus.com/inward/record.url?scp=80052289022&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052289022&partnerID=8YFLogxK

U2 - 10.1016/j.jip.2011.07.004

DO - 10.1016/j.jip.2011.07.004

M3 - Article

C2 - 21843526

AN - SCOPUS:80052289022

SN - 0022-2011

VL - 108

SP - 126

EP - 130

JO - Journal of Invertebrate Pathology

JF - Journal of Invertebrate Pathology

IS - 2

ER -

Comparative analysis of hemocyte phagocytosis between six species of arthropods as measured by flow cytometry

Abstract

Bibliographical note

Keywords

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this