Comparative transcriptional profiling and preliminary study on heterosis mechanism of super-hybrid rice

Gui Sheng Song; Hong Li Zhai; Yong Gang Peng; Lei Zhang; Gang Wei; Xiao Ying Chen; Yu Guo Xiao; Lili Wang; Yue Jun Chen; Bin Wu; Bin Chen; Yu Zhang; Hua Chen; Xiu Jing Feng; Wan Kui Gong; Yao Liu; Zhi Jie Yin; Feng Wang; Guo Zhen Liu; Hong Lin Xu; Xiao Li Wei; Xiao Ling Zhao; Pieter B.F. Ouwerkerk; Thomas Hankemeier; Theo Reijmers; Rob Van Der Heijden; Cong Ming Lu; Mei Wang; Jan Van Der Greef; Zhen Zhu

doi:10.1093/mp/ssq046

Comparative transcriptional profiling and preliminary study on heterosis mechanism of super-hybrid rice

Gui Sheng Song, Hong Li Zhai, Yong Gang Peng, Lei Zhang, Gang Wei, Xiao Ying Chen, Yu Guo Xiao, Lili Wang, Yue Jun Chen, Bin Wu, Bin Chen, Yu Zhang, Hua Chen, Xiu Jing Feng, Wan Kui Gong, Yao Liu, Zhi Jie Yin, Feng Wang, Guo Zhen Liu, Hong Lin XuXiao Li Wei, Xiao Ling Zhao, Pieter B.F. Ouwerkerk, Thomas Hankemeier, Theo Reijmers, Rob Van Der Heijden, Cong Ming Lu, Mei Wang, Jan Van Der Greef, Zhen Zhu

Research output: Contribution to journal › Article › peer-review

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Abstract

Heterosis is a biological phenomenon whereby the offspring from two parents show improved and superior performance than either inbred parental lines. Hybrid rice is one of the most successful apotheoses in crops utilizing heterosis. Transcriptional profiling of F1 super-hybrid rice Liangyou-2186 and its parents by serial analysis of gene expression (SAGE) revealed 1183 differentially expressed genes (DGs), among which DGs were found significantly enriched in pathways such as photosynthesis and carbon-fixation, and most of the key genes involved in the carbon-fixation pathway exhibited up-regulated expression in F1 hybrid rice. Moreover, increased catabolic activity of corresponding enzymes and photosynthetic efficiency were also detected, which combined to indicate that carbon fixation is enhanced in F1 hybrid, and might probably be associated with the yield vigor and heterosis in super-hybrid rice. By correlating DGs with yield-related quantitative trait loci (QTL), a potential relationship between differential gene expression and phenotypic changes was also found. In addition, a regulatory network involving circadian-rhythms and light signaling pathways was also found, as previously reported in Arabidopsis, which suggest that such a network might also be related with heterosis in hybrid rice. Altogether, the present study provides another view for understanding the molecular mechanism underlying heterosis in rice.

Original language	English (US)
Pages (from-to)	1012-1025
Number of pages	14
Journal	Molecular Plant
Volume	3
Issue number	6
DOIs	https://doi.org/10.1093/mp/ssq046
State	Published - Nov 2010
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the grants from the Major Project of China on New varieties of GMO Cultivation (2008ZX08001-001, 2008ZX08012-002), the Research Project on Rice Functional Genes related to the Yield Performance of the High Technology Research and Development Program (863) of China ( 2006AA10A101 ), the National Program on Key Basic Research Projects (973) of China ( 2004CB720406 , 2007CB109000 ), the Innovation Foundation of Chinese Academy of Science ( KSCX2-SW-306 and KSCX1-SW-03 ), the Program for Strategic Scientific Alliances between China and the Netherlands ( KNAW-PSA04-PSA-BD-04 for P.B.F.O. and M.W., KNAW-CEP project 04CDP022 for Y.X., and the CAS-KNAW Joint PhD Training Programme (05-PhD-02) for Y.Z. Funding to pay the Open Access publication charges for this article was provided by the Major Project of China on New varieties of GMO Cultivation (2008ZX08001-001).

Keywords

Heterosis
carbon fixation
photosynthesis
regulatory network
super-hybrid rice
transcriptional profiling

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Song, G. S., Zhai, H. L., Peng, Y. G., Zhang, L., Wei, G., Chen, X. Y., Xiao, Y. G., Wang, L., Chen, Y. J., Wu, B., Chen, B., Zhang, Y., Chen, H., Feng, X. J., Gong, W. K., Liu, Y., Yin, Z. J., Wang, F., Liu, G. Z., ... Zhu, Z. (2010). Comparative transcriptional profiling and preliminary study on heterosis mechanism of super-hybrid rice. Molecular Plant, 3(6), 1012-1025. https://doi.org/10.1093/mp/ssq046

Song, GS, Zhai, HL, Peng, YG, Zhang, L, Wei, G, Chen, XY, Xiao, YG, Wang, L, Chen, YJ, Wu, B, Chen, B, Zhang, Y, Chen, H, Feng, XJ, Gong, WK, Liu, Y, Yin, ZJ, Wang, F, Liu, GZ, Xu, HL, Wei, XL, Zhao, XL, Ouwerkerk, PBF, Hankemeier, T, Reijmers, T, Van Der Heijden, R, Lu, CM, Wang, M, Van Der Greef, J & Zhu, Z 2010, 'Comparative transcriptional profiling and preliminary study on heterosis mechanism of super-hybrid rice', Molecular Plant, vol. 3, no. 6, pp. 1012-1025. https://doi.org/10.1093/mp/ssq046

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title = "Comparative transcriptional profiling and preliminary study on heterosis mechanism of super-hybrid rice",

abstract = "Heterosis is a biological phenomenon whereby the offspring from two parents show improved and superior performance than either inbred parental lines. Hybrid rice is one of the most successful apotheoses in crops utilizing heterosis. Transcriptional profiling of F1 super-hybrid rice Liangyou-2186 and its parents by serial analysis of gene expression (SAGE) revealed 1183 differentially expressed genes (DGs), among which DGs were found significantly enriched in pathways such as photosynthesis and carbon-fixation, and most of the key genes involved in the carbon-fixation pathway exhibited up-regulated expression in F1 hybrid rice. Moreover, increased catabolic activity of corresponding enzymes and photosynthetic efficiency were also detected, which combined to indicate that carbon fixation is enhanced in F1 hybrid, and might probably be associated with the yield vigor and heterosis in super-hybrid rice. By correlating DGs with yield-related quantitative trait loci (QTL), a potential relationship between differential gene expression and phenotypic changes was also found. In addition, a regulatory network involving circadian-rhythms and light signaling pathways was also found, as previously reported in Arabidopsis, which suggest that such a network might also be related with heterosis in hybrid rice. Altogether, the present study provides another view for understanding the molecular mechanism underlying heterosis in rice.",

keywords = "Heterosis, carbon fixation, photosynthesis, regulatory network, super-hybrid rice, transcriptional profiling",

author = "Song, {Gui Sheng} and Zhai, {Hong Li} and Peng, {Yong Gang} and Lei Zhang and Gang Wei and Chen, {Xiao Ying} and Xiao, {Yu Guo} and Lili Wang and Chen, {Yue Jun} and Bin Wu and Bin Chen and Yu Zhang and Hua Chen and Feng, {Xiu Jing} and Gong, {Wan Kui} and Yao Liu and Yin, {Zhi Jie} and Feng Wang and Liu, {Guo Zhen} and Xu, {Hong Lin} and Wei, {Xiao Li} and Zhao, {Xiao Ling} and Ouwerkerk, {Pieter B.F.} and Thomas Hankemeier and Theo Reijmers and {Van Der Heijden}, Rob and Lu, {Cong Ming} and Mei Wang and {Van Der Greef}, Jan and Zhen Zhu",

note = "Funding Information: This work was supported by the grants from the Major Project of China on New varieties of GMO Cultivation (2008ZX08001-001, 2008ZX08012-002), the Research Project on Rice Functional Genes related to the Yield Performance of the High Technology Research and Development Program (863) of China ( 2006AA10A101 ), the National Program on Key Basic Research Projects (973) of China ( 2004CB720406 , 2007CB109000 ), the Innovation Foundation of Chinese Academy of Science ( KSCX2-SW-306 and KSCX1-SW-03 ), the Program for Strategic Scientific Alliances between China and the Netherlands ( KNAW-PSA04-PSA-BD-04 for P.B.F.O. and M.W., KNAW-CEP project 04CDP022 for Y.X., and the CAS-KNAW Joint PhD Training Programme (05-PhD-02) for Y.Z. Funding to pay the Open Access publication charges for this article was provided by the Major Project of China on New varieties of GMO Cultivation (2008ZX08001-001). ",

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AU - Zhai, Hong Li

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AU - Wei, Gang

AU - Chen, Xiao Ying

AU - Xiao, Yu Guo

AU - Wang, Lili

AU - Chen, Yue Jun

AU - Wu, Bin

AU - Chen, Bin

AU - Zhang, Yu

AU - Chen, Hua

AU - Feng, Xiu Jing

AU - Gong, Wan Kui

AU - Liu, Yao

AU - Yin, Zhi Jie

AU - Wang, Feng

AU - Liu, Guo Zhen

AU - Xu, Hong Lin

AU - Wei, Xiao Li

AU - Zhao, Xiao Ling

AU - Ouwerkerk, Pieter B.F.

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N2 - Heterosis is a biological phenomenon whereby the offspring from two parents show improved and superior performance than either inbred parental lines. Hybrid rice is one of the most successful apotheoses in crops utilizing heterosis. Transcriptional profiling of F1 super-hybrid rice Liangyou-2186 and its parents by serial analysis of gene expression (SAGE) revealed 1183 differentially expressed genes (DGs), among which DGs were found significantly enriched in pathways such as photosynthesis and carbon-fixation, and most of the key genes involved in the carbon-fixation pathway exhibited up-regulated expression in F1 hybrid rice. Moreover, increased catabolic activity of corresponding enzymes and photosynthetic efficiency were also detected, which combined to indicate that carbon fixation is enhanced in F1 hybrid, and might probably be associated with the yield vigor and heterosis in super-hybrid rice. By correlating DGs with yield-related quantitative trait loci (QTL), a potential relationship between differential gene expression and phenotypic changes was also found. In addition, a regulatory network involving circadian-rhythms and light signaling pathways was also found, as previously reported in Arabidopsis, which suggest that such a network might also be related with heterosis in hybrid rice. Altogether, the present study provides another view for understanding the molecular mechanism underlying heterosis in rice.

AB - Heterosis is a biological phenomenon whereby the offspring from two parents show improved and superior performance than either inbred parental lines. Hybrid rice is one of the most successful apotheoses in crops utilizing heterosis. Transcriptional profiling of F1 super-hybrid rice Liangyou-2186 and its parents by serial analysis of gene expression (SAGE) revealed 1183 differentially expressed genes (DGs), among which DGs were found significantly enriched in pathways such as photosynthesis and carbon-fixation, and most of the key genes involved in the carbon-fixation pathway exhibited up-regulated expression in F1 hybrid rice. Moreover, increased catabolic activity of corresponding enzymes and photosynthetic efficiency were also detected, which combined to indicate that carbon fixation is enhanced in F1 hybrid, and might probably be associated with the yield vigor and heterosis in super-hybrid rice. By correlating DGs with yield-related quantitative trait loci (QTL), a potential relationship between differential gene expression and phenotypic changes was also found. In addition, a regulatory network involving circadian-rhythms and light signaling pathways was also found, as previously reported in Arabidopsis, which suggest that such a network might also be related with heterosis in hybrid rice. Altogether, the present study provides another view for understanding the molecular mechanism underlying heterosis in rice.

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KW - photosynthesis

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KW - super-hybrid rice

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JO - Molecular Plant

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ER -

Comparative transcriptional profiling and preliminary study on heterosis mechanism of super-hybrid rice

Abstract

Bibliographical note

Keywords

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