Comparing compound-specific and bulk stable nitrogen isotope trophic discrimination factors across multiple freshwater fish species and diets

Chelsey M. Blanke; Yoshito Chikaraishi; Yuko Takizawa; Shawn A. Steffan; Prarthana S. Dharampal; M. Jake Vander Zanden

doi:10.1139/cjfas-2016-0420

Comparing compound-specific and bulk stable nitrogen isotope trophic discrimination factors across multiple freshwater fish species and diets

Chelsey M. Blanke, Yoshito Chikaraishi, Yuko Takizawa, Shawn A. Steffan, Prarthana S. Dharampal, M. Jake Vander Zanden

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Compound-specific nitrogen stable isotope analysis provides an approach for estimating animal trophic position that may overcome key issues associated with stable isotope analysis of bulk tissue. Yet compound-specific trophic discrimination factors have not been estimated for a broad range of habitats, taxa, and diets. We conducted a controlled-feeding experiment to characterize the variation in compound-specific (TDF_AA) and bulk (TDFBulk) trophic discrimination factors of four freshwater fish species fed on three distinct diets. We also compared TDF_AA of fish muscle and scale to evaluate the viability of scales for making food web inferences. Mean ±1 SDTDFBulk was 2.2‰ ± 0.9‰, and there were significant effects of species and diet trophic position on TDFBulk. Mean ± 1 SD TDFAA was 6.9‰ ± 0.8‰. Although there was no effect of species on TDF_AA, there were significant differences in TDF_AA across the three diets. TDF_AA from fish scales were not significantly different from those of muscle. Our study illustrates the advantages of estimating trophic position using compound-specific stable isotopes and the need for continued investigation of factors resulting in variation in TDF values.

Original language	English (US)
Pages (from-to)	1291-1297
Number of pages	7
Journal	Canadian Journal of Fisheries and Aquatic Sciences
Volume	74
Issue number	8
DOIs	https://doi.org/10.1139/cjfas-2016-0420
State	Published - 2017
Externally published	Yes

Bibliographical note

Funding Information:
We thank S. Long, M. Messing, T. Barry, and M. Freitas for assisting in the maintenance and cleaning of the aquarium system and feeding of the experimental fishes. M. Freitas and M. Wolman respectively provided the fathead minnows and zebrafish used in the feeding study, and J. Walsh and Z. DeQuatro assisted in initial aquarium system construction. P. Ostrom, N. Ostrom, and K. Morra shared their knowledge and expertise in the planning stages of this work. J. Pauli, S. Larson, T. Tunney, E. Pedersen, J. Walsh, A. Mikulyuk, C. Smith, M. Perales, and D. Goto provided helpful suggestions on earlier versions of the manuscript. This work was funded by the University of Wisconsin Sea Grant (Federal Grants Nos. HCE-7 and HCE-24, Projects PRJ57MH and PRJ87XV awarded to M. Jake Vander Zanden).

Publisher Copyright:
© 2017, Canadian Science Publishing. All rights reserved.

Access

10.1139/cjfas-2016-0420

http://hdl.handle.net/1807/77063

OpenUrl availability

Full text

Cite this

@article{d83c001d28b54838bf18d48b2d327046,

title = "Comparing compound-specific and bulk stable nitrogen isotope trophic discrimination factors across multiple freshwater fish species and diets",

abstract = "Compound-specific nitrogen stable isotope analysis provides an approach for estimating animal trophic position that may overcome key issues associated with stable isotope analysis of bulk tissue. Yet compound-specific trophic discrimination factors have not been estimated for a broad range of habitats, taxa, and diets. We conducted a controlled-feeding experiment to characterize the variation in compound-specific (TDFAA) and bulk (TDFBulk) trophic discrimination factors of four freshwater fish species fed on three distinct diets. We also compared TDFAA of fish muscle and scale to evaluate the viability of scales for making food web inferences. Mean ±1 SDTDFBulk was 2.2‰ ± 0.9‰, and there were significant effects of species and diet trophic position on TDFBulk. Mean ± 1 SD TDFAA was 6.9‰ ± 0.8‰. Although there was no effect of species on TDFAA, there were significant differences in TDFAA across the three diets. TDFAA from fish scales were not significantly different from those of muscle. Our study illustrates the advantages of estimating trophic position using compound-specific stable isotopes and the need for continued investigation of factors resulting in variation in TDF values.",

author = "Blanke, {Chelsey M.} and Yoshito Chikaraishi and Yuko Takizawa and Steffan, {Shawn A.} and Dharampal, {Prarthana S.} and {Vander Zanden}, {M. Jake}",

note = "Funding Information: We thank S. Long, M. Messing, T. Barry, and M. Freitas for assisting in the maintenance and cleaning of the aquarium system and feeding of the experimental fishes. M. Freitas and M. Wolman respectively provided the fathead minnows and zebrafish used in the feeding study, and J. Walsh and Z. DeQuatro assisted in initial aquarium system construction. P. Ostrom, N. Ostrom, and K. Morra shared their knowledge and expertise in the planning stages of this work. J. Pauli, S. Larson, T. Tunney, E. Pedersen, J. Walsh, A. Mikulyuk, C. Smith, M. Perales, and D. Goto provided helpful suggestions on earlier versions of the manuscript. This work was funded by the University of Wisconsin Sea Grant (Federal Grants Nos. HCE-7 and HCE-24, Projects PRJ57MH and PRJ87XV awarded to M. Jake Vander Zanden). Publisher Copyright: {\textcopyright} 2017, Canadian Science Publishing. All rights reserved.",

year = "2017",

doi = "10.1139/cjfas-2016-0420",

language = "English (US)",

volume = "74",

pages = "1291--1297",

journal = "Canadian Journal of Fisheries and Aquatic Sciences",

issn = "0706-652X",

publisher = "National Research Council of Canada",

number = "8",

}

TY - JOUR

T1 - Comparing compound-specific and bulk stable nitrogen isotope trophic discrimination factors across multiple freshwater fish species and diets

AU - Blanke, Chelsey M.

AU - Chikaraishi, Yoshito

AU - Takizawa, Yuko

AU - Steffan, Shawn A.

AU - Dharampal, Prarthana S.

AU - Vander Zanden, M. Jake

N1 - Funding Information: We thank S. Long, M. Messing, T. Barry, and M. Freitas for assisting in the maintenance and cleaning of the aquarium system and feeding of the experimental fishes. M. Freitas and M. Wolman respectively provided the fathead minnows and zebrafish used in the feeding study, and J. Walsh and Z. DeQuatro assisted in initial aquarium system construction. P. Ostrom, N. Ostrom, and K. Morra shared their knowledge and expertise in the planning stages of this work. J. Pauli, S. Larson, T. Tunney, E. Pedersen, J. Walsh, A. Mikulyuk, C. Smith, M. Perales, and D. Goto provided helpful suggestions on earlier versions of the manuscript. This work was funded by the University of Wisconsin Sea Grant (Federal Grants Nos. HCE-7 and HCE-24, Projects PRJ57MH and PRJ87XV awarded to M. Jake Vander Zanden). Publisher Copyright: © 2017, Canadian Science Publishing. All rights reserved.

PY - 2017

Y1 - 2017

N2 - Compound-specific nitrogen stable isotope analysis provides an approach for estimating animal trophic position that may overcome key issues associated with stable isotope analysis of bulk tissue. Yet compound-specific trophic discrimination factors have not been estimated for a broad range of habitats, taxa, and diets. We conducted a controlled-feeding experiment to characterize the variation in compound-specific (TDFAA) and bulk (TDFBulk) trophic discrimination factors of four freshwater fish species fed on three distinct diets. We also compared TDFAA of fish muscle and scale to evaluate the viability of scales for making food web inferences. Mean ±1 SDTDFBulk was 2.2‰ ± 0.9‰, and there were significant effects of species and diet trophic position on TDFBulk. Mean ± 1 SD TDFAA was 6.9‰ ± 0.8‰. Although there was no effect of species on TDFAA, there were significant differences in TDFAA across the three diets. TDFAA from fish scales were not significantly different from those of muscle. Our study illustrates the advantages of estimating trophic position using compound-specific stable isotopes and the need for continued investigation of factors resulting in variation in TDF values.

AB - Compound-specific nitrogen stable isotope analysis provides an approach for estimating animal trophic position that may overcome key issues associated with stable isotope analysis of bulk tissue. Yet compound-specific trophic discrimination factors have not been estimated for a broad range of habitats, taxa, and diets. We conducted a controlled-feeding experiment to characterize the variation in compound-specific (TDFAA) and bulk (TDFBulk) trophic discrimination factors of four freshwater fish species fed on three distinct diets. We also compared TDFAA of fish muscle and scale to evaluate the viability of scales for making food web inferences. Mean ±1 SDTDFBulk was 2.2‰ ± 0.9‰, and there were significant effects of species and diet trophic position on TDFBulk. Mean ± 1 SD TDFAA was 6.9‰ ± 0.8‰. Although there was no effect of species on TDFAA, there were significant differences in TDFAA across the three diets. TDFAA from fish scales were not significantly different from those of muscle. Our study illustrates the advantages of estimating trophic position using compound-specific stable isotopes and the need for continued investigation of factors resulting in variation in TDF values.

UR - http://www.scopus.com/inward/record.url?scp=85018198206&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85018198206&partnerID=8YFLogxK

U2 - 10.1139/cjfas-2016-0420

DO - 10.1139/cjfas-2016-0420

M3 - Article

AN - SCOPUS:85018198206

SN - 0706-652X

VL - 74

SP - 1291

EP - 1297

JO - Canadian Journal of Fisheries and Aquatic Sciences

JF - Canadian Journal of Fisheries and Aquatic Sciences

IS - 8

ER -

Comparing compound-specific and bulk stable nitrogen isotope trophic discrimination factors across multiple freshwater fish species and diets

Abstract

Bibliographical note

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this