Fungal communities associated with plant tissues were compared between two bryophyte species dominating decaying logs (Scapania bolanderi and Pleurozium schreberi), and roots of spruce seedlings growing on the bryophytes and in the ground soil, to evaluate the contribution of fungal communities to seedling regeneration. Using high-throughput DNA sequencing, a total of 1233 fungal operational taxonomic units (OTUs) were detected. Saprotrophic Ascomycota were dominant in bryophytes, whereas ectomycorrhizal (ECM) Basidiomycota were dominant in spruce roots. Fungal communities were significantly different between the two bryophyte species. In addition, fungal communities of spruce seedlings were significantly affected by the substrates on which they were growing. Some ECM fungi were detected from both of the bryophytes and the spruce seedlings growing on them; however, the dominant OTU identities differed between the two bryophyte systems. The possible effects of functional differences between dominant fungal OTUs on spruce seedling regeneration are discussed.
Bibliographical noteFunding Information:
The authors are grateful to Akira S. Mori for providing study site. We also thank Yu Matsuki for help in running high-throughput DNA sequencer. Thanks are extended to Kenji Seiwa, Yoshihisa Suyama, Chika Mitsuyuki, Yoshihiro Tsunamoto and members of the laboratory of Forest Ecology, Tohoku University for valuable discussions. We are also grateful to Emma Gilmartin for checking English of the draft manuscript and two anonimous reviewers and handling editor Marie Louise Davey for their constructive comments which improve draft manuscript greatly. This study was financed by Japan Society for Promotion of Science (JSPS) KAKENHI Grant Number 26850093 to YF.
- Coarse woody debris
- Fungal community
- Picea jezoensis var. hondoensis
- Seedling regeneration