Comparison of low- and high-calcium-requiring forms of the calcium-activated protease with their autocatalytic breakdown products

W. R. Dayton

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63 Scopus citations

Abstract

The effect of controlled autocatalytic degradation on the subunit molecular weight, subunit composition and calcium sensitivity of the low-calcium-requiring and the high-calcium-requiring forms of the calcium-activated muscle protease was examined. Purified low- and high-calcium-requiring proteases coelectrophorese on SDS-polyacrylamide gels. Controlled autocatalysis of either form of the protease results in extensive degradation of their respective 30 kDa subunits and partial degradation of their 80 kDa subunits. In this electrophoresis system the electrophoretic banding pattern of the low-calcium-requiring protease is clearly different from that of the autocatalytically degraded high-calcium-requiring protease. Similar results were obtained using a nondenaturing polyacrylamide gel system. Both the high- and low-calcium-requiring proteases were made more sensitive to calcium by autocatalytic degradation. However, the results of this study strongly indicate that autocatalytic degradation does not resultin conversion of high-calcium-requiring protease to the low-calcium-requiring protease as has been recently hypothesized (Suzuki et al. (1981) J. Biochem. 90, 275-278).

Original languageEnglish (US)
Pages (from-to)166-172
Number of pages7
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume709
Issue number2
DOIs
StatePublished - Dec 20 1982

Bibliographical note

Funding Information:
ported by grants from the Muscular Dystrophy Association of America, the Minnesota Heart Association and the Minnesota Agricultural Experiment Station. This is Scientific Journal Series Paper No. 13064, Minnesota Agricultural Experiment Station, University of Minnesota, St. Paul, MN 55108, U.S.A.

Keywords

  • (Porcine skeletal muscle)
  • Autocatalysis
  • Ca activation
  • Proteinase
  • Subunit structure

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