Comparison of microscopic and flow cytometric detection of platelet antibody in dogs suspected of having immune-mediated thrombocytopenia.

A. T. Kristensen, D. J. Weiss, J. S. Klausner, J. Laber, D. J. Christie

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

A flow cytometric platelet immunofluorescence assay (FC-PIFA) was compared with a previously developed microscopic platelet immunofluorescence assay (MI-PIFA) for detection of circulating platelet antibody. Both assays were performed on serum from 10 healthy dogs with normal platelet count, and on serum from 27 thrombocytopenic dogs--18 had primary immune-mediated thrombocytopenia (IMT), and 9 had IMT in addition to other immune-mediated disease (secondary IMT). Both assays yielded negative results for all control dogs. The MI-PIFA and FC-PIFA results were in agreement in 23 dogs with IMT (14 positive and 9 negative). There was linear correlation between MI-PIFA scores and FC-PIFA results (r = 0.873). Positive results were obtained for 55.5% of the dogs with suspected IMT, using the MI-PIFA, compared with 67%, using the FC-PIFA; however, the difference was not statistically significant. Use of fresh or frozen fixed donor platelets as the antigen source yielded similar results in the FC-PIFA.

Original languageEnglish (US)
Pages (from-to)1111-1114
Number of pages4
JournalAmerican journal of veterinary research
Volume55
Issue number8
StatePublished - Aug 1994

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