We determined the concentration of the messenger RNA species which encode four (m1-m4) of the five cloned muscarinic receptors in brains of Alzheimer's disease patients as compared to age-matched controls. Assays were performed using the quantitative method of DNA-excess solution hybridization in the cerebral cortex (frontal, temporal and occipital), hippocampus, nucleus basalis of Meynert and brainstem. The results suggest a statistically significant decrease in the m1 muscarinic receptor message in the temporal and occipital cortex, with no change in other regions. There was no change in the level of mRNA encoding the m2, m3 or m4 receptors in any of the brain regions studied.
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Acknowledgements. The experiments included herein were performed while EEE, SZZ and SZW were at the University of Maryland School of Pharmacy. This work was supported in part by NIH Grants NS-25743 and by a contract from the US Army Research Office (DAAL-03-88-K-0078). E.E.E. was supported during the course of the work by a Research Career Development Award from NIH (AG-00344). The University of Miami Brain Endowment Bank is funded in part by the National Parkinson Foundation, Miami, FL and the GRECC, Veterans Administration Medical Center, Miami, FL. The procedures for autopsy have been funded by the National Institute on Aging (AG-05128-09S1).
- Alzheimer's disease
- Muscarinic receptor
- Receptor subtype