The cytotoxicity of 5-thio-D-glucose (5-TG) and misonidazole (MISO) on hypoxic cells in vitro was compared, and the combined effect of these two compounds was investigated. The cytotoxicity of 5-TG on hypoxic SCK mammary carcinoma cells of mice was markedly greater than that of MISO. However, MISO was more toxic than was 5-TG to P815-X2 mastocytoma cells of mice. The elevation of temperature from 37.5°C to 40.0°C during incubation with drugs greatly enhanced the cell death. The thermal enhancement of cytotoxicity of 5-TG to mastocytoma cells appeared to be greater than that of MISO. Preliminary studies indicated that the combined effect of 5-TG and MISO is additive, suggesting the intracellular targets or cytocidal mechanisms of these two compounds may be different.
|Original language||English (US)|
|Number of pages||4|
|Journal||International Journal of Radiation Oncology, Biology, Physics|
|State||Published - 1982|
Bibliographical noteFunding Information:
This work was supported by a grant (#CA 2 I28 1) awarded by the NCI. Reprint requests to: C.vV. Song, Ph.D., University of Minnesota, Department of Therapeutic Radiology, Box 494 Mayo Hospital, Minneapolis, MN 55455. Presented at the CROS Conference on Chemical Modifica-
- Hypoxic cells