Complex microcolinearity among wheat, rice, and barley revealed by fine mapping of the genomic region harboring a major QTL for resistance to Fusarium head blight in wheat

Sixin Liu; Xiuling Zhang; Michael O. Pumphrey; Robert W. Stack; Bikram S. Gill; James A. Anderson

doi:10.1007/s10142-005-0007-y

Complex microcolinearity among wheat, rice, and barley revealed by fine mapping of the genomic region harboring a major QTL for resistance to Fusarium head blight in wheat

Sixin Liu, Xiuling Zhang, Michael O. Pumphrey, Robert W. Stack, Bikram S. Gill, James A. Anderson

Agronomy and Plant Genetics

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Abstract

A major quantitative trait locus (QTL), Qfhs.ndsu-3BS, for resistance to Fusarium head blight (FHB) in wheat has been identified and verified by several research groups. The objectives of this study were to construct a fine genetic map of this QTL region and to examine microcolinearity in the QTL region among wheat, rice, and barley. Two simple sequence repeat (SSR) markers (Xgwm533 and Xgwm493) flanking this QTL were used to screen for recombinants in a population of 3,156 plants derived from a single F₇ plant heterozygous for the Qfhs.ndsu-3BS region. A total of 382 recombinants were identified, and they were genotyped with two more SSR markers and eight sequence-tagged site (STS) markers. A fine genetic map of the Qfhs.ndsu-3BS region was constructed and spanned 6.3 cM. Based on replicated evaluations of homozygous recombinant lines for Type II FHB resistance, Qfhs.ndsu-3BS, redesignated as Fhb1, was placed into a 1.2-cM marker interval flanked by STS3B-189 and STS3B-206. Primers of STS markers were designed from wheat expressed sequence tags homologous to each of six barley genes expected to be located near this QTL region. A comparison of the wheat fine genetic map and physical maps of rice and barley revealed inversions and insertions/deletions. This suggests a complex microcolinearity among wheat, rice, and barley in this QTL region.

Original language	English (US)
Pages (from-to)	83-89
Number of pages	7
Journal	Functional and Integrative Genomics
Volume	6
Issue number	2
DOIs	https://doi.org/10.1007/s10142-005-0007-y
State	Published - Mar 2006

Bibliographical note

Funding Information:
Acknowledgements The authors would like to thank Gary Linkert, Roger Fuentes, Aaron Lorenz, and Greg Snyder for their technical assistance. This project was supported by the National Research Initiative of the USDA Cooperative State Research, Education, and Extension Service, grant number 2004-35300-14787, and the US Department of Agriculture, under agreement No. 59-0790-0025. This is a cooperative project with the US Wheat and Barley Scab Initiative.

Keywords

Barley
Fusarium head blight
Microcolinearity
Rice
Wheat

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s10142-005-0007-y

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Complex microcolinearity among wheat, rice, and barley revealed by fine mapping of the genomic region harboring a major QTL for resistance to Fusarium head blight in wheat. / Liu, Sixin; Zhang, Xiuling; Pumphrey, Michael O. et al.
In: Functional and Integrative Genomics, Vol. 6, No. 2, 03.2006, p. 83-89.

Research output: Contribution to journal › Article › peer-review

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abstract = "A major quantitative trait locus (QTL), Qfhs.ndsu-3BS, for resistance to Fusarium head blight (FHB) in wheat has been identified and verified by several research groups. The objectives of this study were to construct a fine genetic map of this QTL region and to examine microcolinearity in the QTL region among wheat, rice, and barley. Two simple sequence repeat (SSR) markers (Xgwm533 and Xgwm493) flanking this QTL were used to screen for recombinants in a population of 3,156 plants derived from a single F7 plant heterozygous for the Qfhs.ndsu-3BS region. A total of 382 recombinants were identified, and they were genotyped with two more SSR markers and eight sequence-tagged site (STS) markers. A fine genetic map of the Qfhs.ndsu-3BS region was constructed and spanned 6.3 cM. Based on replicated evaluations of homozygous recombinant lines for Type II FHB resistance, Qfhs.ndsu-3BS, redesignated as Fhb1, was placed into a 1.2-cM marker interval flanked by STS3B-189 and STS3B-206. Primers of STS markers were designed from wheat expressed sequence tags homologous to each of six barley genes expected to be located near this QTL region. A comparison of the wheat fine genetic map and physical maps of rice and barley revealed inversions and insertions/deletions. This suggests a complex microcolinearity among wheat, rice, and barley in this QTL region.",

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Complex microcolinearity among wheat, rice, and barley revealed by fine mapping of the genomic region harboring a major QTL for resistance to Fusarium head blight in wheat

Abstract

Bibliographical note

Keywords

UN SDGs

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