Complexation of the nickel and cobalt transcriptional regulator RcnR with DNA

Chao Li; Joseph W. Vavra; Carolyn E. Carr; Hsin Ting Huang; Michael J. Maroney; Carrie M. Wilmot

doi:10.1107/S2053230X19017084

Complexation of the nickel and cobalt transcriptional regulator RcnR with DNA

Chao Li, Joseph W. Vavra, Carolyn E. Carr, Hsin Ting Huang, Michael J. Maroney, Carrie M. Wilmot

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Abstract

RcnR is a transcription factor that regulates the homeostasis of cobalt and nickel in bacterial cells. Escherichia coli RcnR was crystallized with DNA that encompasses the DNA-binding site. X-ray diffraction data were collected to 2.9Å resolution. The crystal belonged to space group P6122 or P6522, with unit-cell parameters a = b = 73.59, c = 157.66Å, α = β = 90, γ = 120°.

Original language	English (US)
Pages (from-to)	25-30
Number of pages	6
Journal	Acta Crystallographica Section F: Structural Biology Communications
Volume	76
DOIs	https://doi.org/10.1107/S2053230X19017084
State	Published - Jan 1 2020

Bibliographical note

Funding Information:
The final X-ray diffraction data were collected at Argonne National Laboratory, Sector 23, GM/CA at the Advanced Photon Source. Argonne is operated by UChicago Argonne LLC for the US Department of Energy, Office of Biological and Environmental Research under contract DE-AC02-06CH11357. GM/CA@APS has been funded in whole or in part with Federal funds from the National Cancer Institute (ACB-12002) and the National Institute of General Medical Sciences (AGM-12006). The EIGER 16M detector was funded by an NIH–Office of Research Infrastructure Programs High-End Instrumentation Grant (S10 OD012289). We thank Nagarajan Venugopalan at APS 23-ID-B for the help during data collection. Computational resources and software were made available by the Minnesota Supercomputing Institute. Preliminary X-ray diffraction data were collected at the Kahlert Structural Biology Laboratory, University of Minnesota, and we thank Ed Hoeffner for his technical support. We also thank Dr Hideki Aihara and Dr Ke Shi for valuable discussions.

Funding Information:
This work was supported by a grant from the National Institutes of Health R01-GM069696 (MJM).

Publisher Copyright:
© 2020 International Union of Crystallography.

Keywords

RcnR
cobalt homeostasis
nickel homeostasis
transcription factor

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abstract = "RcnR is a transcription factor that regulates the homeostasis of cobalt and nickel in bacterial cells. Escherichia coli RcnR was crystallized with DNA that encompasses the DNA-binding site. X-ray diffraction data were collected to 2.9{\AA} resolution. The crystal belonged to space group P6122 or P6522, with unit-cell parameters a = b = 73.59, c = 157.66{\AA}, α = β = 90, γ = 120°.",

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Complexation of the nickel and cobalt transcriptional regulator RcnR with DNA

Abstract

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