TY - JOUR
T1 - Conformational and receptor binding properties of human EGF and TGF‐α second loop fragments
AU - Han, Kyou‐Hoon ‐H
AU - Ferretti, James A.
AU - Niu, Chien‐Hua ‐H
AU - Lokeshwar, Vinatu
AU - Clarke, Robert
AU - Katz, Deborah
PY - 1988/6
Y1 - 1988/6
N2 - The solution conformation of the second loop fragment of human EGF, [Ala20]EGF(14–31), was determined using two‐dimensional NMR homonuclear Hartmann–Hahn and rotating frame nuclear Overhauser enhancement spectroscopy. The results are compared with the conformation of the second loop fragment of human TGF‐α, [Ala21] TGF‐α(16–32), and with that of the second loop of intact EGF. Comparison of the two experimentally determined structures of the second loop fragments shows significant differences in the turn regions of each peptide. For the EGF fragment, hydrophobic side chain groups protrude away from the ring, whereas for the TGF‐α fragment hydrophilic groups are directed away from the ring. Although these turn regions represent the putative receptor binding sites, neither second loop fragment binds to the EGF receptor. The biological activity is discussed in terms of the conformational differences found for the two second loop fragments.
AB - The solution conformation of the second loop fragment of human EGF, [Ala20]EGF(14–31), was determined using two‐dimensional NMR homonuclear Hartmann–Hahn and rotating frame nuclear Overhauser enhancement spectroscopy. The results are compared with the conformation of the second loop fragment of human TGF‐α, [Ala21] TGF‐α(16–32), and with that of the second loop of intact EGF. Comparison of the two experimentally determined structures of the second loop fragments shows significant differences in the turn regions of each peptide. For the EGF fragment, hydrophobic side chain groups protrude away from the ring, whereas for the TGF‐α fragment hydrophilic groups are directed away from the ring. Although these turn regions represent the putative receptor binding sites, neither second loop fragment binds to the EGF receptor. The biological activity is discussed in terms of the conformational differences found for the two second loop fragments.
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U2 - 10.1002/jmr.300010304
DO - 10.1002/jmr.300010304
M3 - Article
C2 - 3273222
AN - SCOPUS:0024020582
SN - 0952-3499
VL - 1
SP - 116
EP - 123
JO - Journal of Molecular Recognition
JF - Journal of Molecular Recognition
IS - 3
ER -