Conservation of interphase chromatin nonhistone antigens as components of metaphase chromosomes

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Abstract

The degree of conservation of HeLa interphase chromatin nonhistone antigens among the nonhistones of isolated metaphase chromosomes was determined with immunological procedures. Proteins were separated on SDS-polyacrylamide gels and electrophoretically transferred to diazophenylthioether (DPT)-paper, which was then overlaid with antiserum to chromatin from interphase nuclei. The bound antibodies were detected with 125I-labeled protein A. Alternatively, polyacrylamide gels were directly overlaid with antiserum and with 125I-protein A. Densitometry of autoradiograms and stained gels revealed the degree of conservation of nonhistone antigenic determinants from interphase to metaphase to be over 90% for chromatin.

Original languageEnglish (US)
Pages (from-to)211-215
Number of pages5
JournalFEBS Letters
Volume165
Issue number2
DOIs
StatePublished - Jan 9 1984

Bibliographical note

Funding Information:
This researchw ass upportedb y a grant from the National Instituteso f Health and by funds from the Biomedical Research Support Grant of the University of MinnesotaM edical School.

Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.

Keywords

  • Antiserum
  • Chromatin
  • Electrophoresis
  • HeLa cell
  • Metaphase chromosome
  • Nonhistone

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