Abstract
Purpose: To study the possibility of transferring decorin gene to rat glomerular mesangial cell. Methods: Amplification of the rat decorin (DCN) cDNA by RT-PCR for constructing the plasmid pcDNA3. 1A-DCN and lipofectin method for transfecting DCN gene into MsC; G418 scanning, Western blot and RT-PCR analysis for detecting DCN protein and mRNA in D-A6 cell clone. Results: The recombinant eukaryotic expression plasmid, pcDNA3. 1A-DCN was successfully constructed and 2 cell clones positively expressing DCN were selected. Conclusions: These cell clones positively expressing DCN is valuable for providing favorable experimental bases of gene therapy to model with glomerular diseases.
Original language | English (US) |
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Pages (from-to) | 97-99+102 |
Journal | Fudan University Journal of Medical Sciences |
Volume | 28 |
Issue number | 2 |
State | Published - Dec 1 2001 |
Keywords
- Decorin
- Gene transfection
- Mesangial cell