Purpose: To study the possibility of transferring decorin gene to rat glomerular mesangial cell. Methods: Amplification of the rat decorin (DCN) cDNA by RT-PCR for constructing the plasmid pcDNA3. 1A-DCN and lipofectin method for transfecting DCN gene into MsC; G418 scanning, Western blot and RT-PCR analysis for detecting DCN protein and mRNA in D-A6 cell clone. Results: The recombinant eukaryotic expression plasmid, pcDNA3. 1A-DCN was successfully constructed and 2 cell clones positively expressing DCN were selected. Conclusions: These cell clones positively expressing DCN is valuable for providing favorable experimental bases of gene therapy to model with glomerular diseases.
|Original language||English (US)|
|Journal||Fudan University Journal of Medical Sciences|
|State||Published - Dec 1 2001|
- Gene transfection
- Mesangial cell