Constitutive function of the Ikaros transcription factor in primary leukemia cells from pediatric newly diagnosed high-risk and relapsed B-precursor ALL patients

Fatih M. Uckun, Hong Ma, Rita Ishkhanian, Martha Arellano, Anoush Shahidzadeh, Amanda Termuhlen, Paul S. Gaynon, Sanjive Qazi

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

We examined the constitutive function of the Ikaros (IK) transcription factor in blast cells from pediatric B-precursor acute lymphoblastic leukemia (BPL) patients using multiple assay platforms and bioinformatics tools. We found no evidence of diminished IK expression or function for primary cells from high-risk BPL patients including a Philadelphia chromosome (Ph)+ subset. Relapse clones as well as very aggressive in vivo clonogenic leukemic B-cell precursors isolated from spleens of xenografted NOD/SCID mice that developed overt leukemia after inoculation with primary leukemic cells of patients with BPL invariably and abundantly expressed intact IK protein. These results demonstrate that a lost or diminished IK function is not a characteristic feature of leukemic cells in Ph+ or Ph- high-risk BPL.

Original languageEnglish (US)
Article numbere80732
JournalPloS one
Volume8
Issue number11
DOIs
StatePublished - Nov 20 2013
Externally publishedYes

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