Abstract
Bioactive compounds from plant foods are intensely investigated for effects on disease prevention. β-Glucuronidase/arylsulfatase from Helix pomatia (snail) is commonly used when quantifying exposure to metabolized dietary components. However, we describe here the contamination of multiple formulations of this enzyme preparation with 3,3'-diindolylmethane (DIM), 8-methoxypsoralen (8-MOP), and 5-methoxypsoralen (5-MOP), bioactives from cruciferous and apiaceous vegetables under investigation as putative cancer chemopreventive agents. We identified an Escherichia coli preparation of β-glucuronidase as free from contamination with any of the compounds tested.These results demonstrate the importance of selecting appropriate enzyme preparations when quantifying naturally occurring, trace level compounds in biological fluids.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 188-193 |
| Number of pages | 6 |
| Journal | Food and Chemical Toxicology |
| Volume | 62 |
| DOIs | |
| State | Published - 2013 |
Bibliographical note
Funding Information:Xun Ming, J. Bradley Hochalter, Brock Matter, Peter Villalta. This work was supported in part by P30 CA77598 from the National Institutes of Health, utilizing the University of Minnesota Masonic Cancer Center Analytical Biochemistry shared resource. The project described was supported by Award Number T32CA132670 and by 5K07CA128952-02 from the National Cancer Institute. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Cancer Institute or the National Institutes of Health.
Keywords
- 3,3'-Diindolylmethane
- 5-Methoxypsoralen
- 8-Methoxypsoralen
- Deconjugation enzymes
- Dietary chemoprevention
- Helix pomatia
