TY - JOUR
T1 - Continuous fluorometric assay of phospholipase A2 with pyrene-labeled lecithin as a substrate
AU - Hendrickson, H. Stewart
AU - Rauk, Phillip N
PY - 1981/9/15
Y1 - 1981/9/15
N2 - 1,2-Bis[4-(1-pyreno)butanoyl]-sn-glycero-3-phosphorylcholine was synthesized as a fluorogenic substrate for phospholipase A2. It has a critical micellar concentration of 7.3 μm and gives only excimer fluorescent emission at 480 nm in aqueous micellar dispersion. When hydrolyzed by phospholipase A2, the products give only monomer emission which is monitored best at 382 and 400 nm. Conditions were developed for an assay for phospholipase A2 using this substrate. The assay was sensitive to as little as 8 ng of pure porcine pancreatic phospholipase A2.
AB - 1,2-Bis[4-(1-pyreno)butanoyl]-sn-glycero-3-phosphorylcholine was synthesized as a fluorogenic substrate for phospholipase A2. It has a critical micellar concentration of 7.3 μm and gives only excimer fluorescent emission at 480 nm in aqueous micellar dispersion. When hydrolyzed by phospholipase A2, the products give only monomer emission which is monitored best at 382 and 400 nm. Conditions were developed for an assay for phospholipase A2 using this substrate. The assay was sensitive to as little as 8 ng of pure porcine pancreatic phospholipase A2.
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U2 - 10.1016/0003-2697(81)90401-2
DO - 10.1016/0003-2697(81)90401-2
M3 - Article
C2 - 7316183
AN - SCOPUS:0019812636
VL - 116
SP - 553
EP - 558
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 2
ER -