Streptococcus suis serotype 2 (S. suis 2) is an important pathogen, responsible for diverse diseases in swine and human. In this study, we investigated the role of the glutamine synthetase (GlnA) in the pathogenesis of S. suis 2 in mice. To assess the contribution of glutamine synthetase (GlnA) to the virulence of S. suis 2, an knockout mutant (ΔglnA) unable to produce GlnA was constructed, and the virulence level of wild-type (WT) SC19 and the ΔglnA mutant strain were compared in an in vitro adherence assay and murine infection models. The data showed that ΔglnA mutant exhibited a significant decrease in adherence to the epithelial cells HEp-2. The ΔglnA mutant strain was attenuated and could reduce mortality and morbidity in murine infection models. Furthermore, organ cultures showed that GlnA plays a role in the colonization of the specific organs involved in S. suis infection. Functional complementation of the glnA gene into the knockout mutant ΔglnA or incubated with extracellular glutamine restored its ability to adhere to the epithelial cells HEp-2. These findings suggested that glnA is required for the full virulence in S. suis 2. Therefore, the ΔglnA mutant was considered as an attenuated mutant.
Bibliographical noteFunding Information:
We are grateful to Dr. Sekizaki (National Institute of Animal Health, Japan) for supplying plasmid pSET4s and Ulrike M. Samen (University of Ulm, Germany) for providing us plasmid pAT18 and their precious guiding for correctly using these plasmids. We thank Dr. Ting Fu and Dr. Jinlin Liu for excellent technical assistance. This research is supported by the grant from The National Basic Research Program (also called 973 Program) (No. 2006CB504404), National Natural Science Foundation of China (No. 30600025), Innovation Teams of Ministry of Education (No. IRT0726) and 863 program (2006AA10A206).
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- Glutamine synthetase
- Streptococcus suis serotype 2