Mutant presenilins cause early-onset of familial Alzheimer's disease and render cells vulnerable to apoptosis. Calsenilin/DREAM/KChIP3 is a multifunctional calcium-binding protein that interacts with presenilin and mediates calcium-mediated apoptosis. In the present study, we report that the calsenilin-mediated apoptosis is regulated by presenilin. The expression of calsenilin was highly up-regulated in neuronal cells undergoing Aβ42-triggered cell death. The incidence of calsenilin-mediated apoptosis was diminished in presenilin-1-/- mouse embryonic fibroblast cells or neuronal cells stably expressing a loss-of-function presenilin-1 mutant. On the contrary, an array of familial Alzheimer's disease-associated presenilin mutants (gain-of-function) increased calsenilin-induced cell death. Moreover, γ-secretase inhibitors, including compound E and DAPT, decreased the calsenilin-induced cell death. These results suggest that the pro-apoptotic activity of calsenilin coordinates with presenilin/γ-secretase activity to play a crucial role in the neuronal death of Alzheimer's disease.
|Original language||English (US)|
|Number of pages||5|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - May 23 2003|
Bibliographical noteFunding Information:
We thank Dr. M. Paul Murphy for the gift of compound E and are grateful to Dr. De Strooper for providing PS1 −/− MEF cell. This work was supported by the grant from National Research Laboratory program (to Y. Jung) and Life Phenomena and Function Research Group Program (2000) of the Korean MOST.
- Alzheimer's disease
- Cell death