TY - JOUR
T1 - Copper-containing amine oxidases. Biogenesis and catalysis; a structural perspective
AU - Brazeau, Brian J.
AU - Johnson, Bryan J.
AU - Wilmot, Carrie M.
PY - 2004/8/1
Y1 - 2004/8/1
N2 - This review will focus on how X-ray crystallographic studies of copper-containing amine oxidases have complemented the solution, kinetic, and spectroscopic research on this ubiquitous class of enzymes. These enzymes not only contain a copper ion at the active site, but also a unique organic cofactor, 2,4,5-trihydroxyphenylalanine quinone (TPQ), which is absolutely required for catalysis. Structural data have not only shed light on the catalytic mechanism of the enzyme, which converts primary amines, using molecular oxygen, to aldehydes, ammonia, and hydrogen peroxide, but also on biogenesis of the cofactor. The cofactor is derived from a tyrosine in the enzyme amino acid sequence and requires only the addition of copper(II) and molecular oxygen in a self-processing event.
AB - This review will focus on how X-ray crystallographic studies of copper-containing amine oxidases have complemented the solution, kinetic, and spectroscopic research on this ubiquitous class of enzymes. These enzymes not only contain a copper ion at the active site, but also a unique organic cofactor, 2,4,5-trihydroxyphenylalanine quinone (TPQ), which is absolutely required for catalysis. Structural data have not only shed light on the catalytic mechanism of the enzyme, which converts primary amines, using molecular oxygen, to aldehydes, ammonia, and hydrogen peroxide, but also on biogenesis of the cofactor. The cofactor is derived from a tyrosine in the enzyme amino acid sequence and requires only the addition of copper(II) and molecular oxygen in a self-processing event.
KW - Copper metalloenzyme
KW - Copper-containing amine oxidases
KW - Oxygen activation
KW - Quinone cofactor
KW - TPQ
KW - X-ray crystallography
UR - http://www.scopus.com/inward/record.url?scp=3042550172&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3042550172&partnerID=8YFLogxK
U2 - 10.1016/j.abb.2004.03.034
DO - 10.1016/j.abb.2004.03.034
M3 - Short survey
C2 - 15234266
AN - SCOPUS:3042550172
SN - 0003-9861
VL - 428
SP - 22
EP - 31
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 1
ER -