TY - JOUR
T1 - Corneal epithelial wound healing
T2 - A tissue culture assay on the effect of antibiotics
AU - Nelson, J. D.
AU - Silverman, V.
AU - Lima, P. H.
AU - Beckman, G.
N1 - Funding Information:
ACKNOWLEDGEMENTS Supported by Ramsey Foundation Grants 8417 and 8436, and a grant from Allergan Pharmaceuticals, Irvine, California.
PY - 1990
Y1 - 1990
N2 - The effects of gentamicin, tobramycin and the fluorquinolone, ofloxacin on corneal epithelial wound healing were determined using an in vitro model of wound healing. Wounds were created in rabbit corneal epithelial cultures which were then exposed to various concentrations of the antibiotics. Effects on wound healing were measured by comparing migration rates between 6 and 18 hours and wound areas at various antibiotic concentrations over 36 hours. Gentamicin at concentrations of 0.5, 1.0, 1.5 and 2.0 mg/ml was found to statistically significantly effect wound healing compared to controls. Tobramycin on the other hand was found to have minimal effect on wound healing up to 2.0 mg/ml. Ofloxacin at concentrations ≥0.05 mg/ml had statistically significant effects on wound-healing.
AB - The effects of gentamicin, tobramycin and the fluorquinolone, ofloxacin on corneal epithelial wound healing were determined using an in vitro model of wound healing. Wounds were created in rabbit corneal epithelial cultures which were then exposed to various concentrations of the antibiotics. Effects on wound healing were measured by comparing migration rates between 6 and 18 hours and wound areas at various antibiotic concentrations over 36 hours. Gentamicin at concentrations of 0.5, 1.0, 1.5 and 2.0 mg/ml was found to statistically significantly effect wound healing compared to controls. Tobramycin on the other hand was found to have minimal effect on wound healing up to 2.0 mg/ml. Ofloxacin at concentrations ≥0.05 mg/ml had statistically significant effects on wound-healing.
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U2 - 10.3109/02713689009044524
DO - 10.3109/02713689009044524
M3 - Article
C2 - 2347205
AN - SCOPUS:0025330179
SN - 0271-3683
VL - 9
SP - 277
EP - 285
JO - Current Eye Research
JF - Current Eye Research
IS - 3
ER -