The mixed microbial communities that occur naturally on lignocellulosic feedstocks can provide feedstock-specific enzyme mixtures to saccharify lignocelluloses. Bacterial-fungal communities were enriched from switchgrass bales to deconstruct ammonia-pretreated switchgrass (DSG). Correlation analysis was carried out to elucidate the relationship between microbial decomposition of DSG by these communities, enzymatic activities produced and enzymatic saccharification of DSG using these enzyme mixtures. Results of the analysis showed that β-glucosidase and xylosidase activities limited the extent of microbial deconstruction and enzymatic saccharification of DSG. The results also underlined the importance of ligninase activity for the enzymatic saccharification of pretreated lignocellulosic feedstock. The bacterial-fungal communities developed in this research can be used to produce enzyme mixtures to deconstruct DSG, and the results from the correlation analysis can be used to optimize these enzyme mixtures for efficient saccharification of DSG to produce second-generation biofuels.
Bibliographical noteFunding Information:
U.S. Department of Energy award number DE-FG36-08GO88071 funded this research. The authors acknowledge Dr. Jim Frederick at the Clemson University Pee Dee Research and Education Center, near Florence, SC, where the switchgrass was grown and harvested. This material is based upon work supported by NIFA / USDA under project number SC-1700474 and is Technical Contribution No. 6403 of the Clemson University Experiment Station.
- Bacterial-fungal consortia
- Lignocellulose degradation