Lactate dehydrogenase-elevating virus (LDV) induces poliomyelitis in immunosuppressed C58 mice resulting in fatal paralysis. We have synthesized and cloned cDNA complementary to the LDV genome, and used the cDNA clones as in situ hybridization probes for the detection of LDV RNA in tissue sections. Direct fluorescent antibody staining using IgG from chronically infected mice was used for the detection of LDV antigens. Using these methods, we have detected LDV RNA and antigens in anterior horn neurons of paralyzed mice. The appearance of LDV RNA and antigen positive motor neurons and their location in the spinal cord correlated with the development of paralytic symptoms. No positive neurons were detected in LDV-infected, susceptible mice without signs of paralysis, but some glial cells of the white and gray matter in the spinal cords of these mice were found to contain LDV RNA. These analyses broaden the host cell range of LDV to include neuronal and other cells in the CNS and support the hypothesis of LDV replication in neurons as the cause of poliomyelitis and paralysis.
Bibliographical noteFunding Information:
We thank Dr. A.T. Haasef or makingh is laboratoryf acilitiesa vailablef or the in situ hybridizations tudies,a nd for advicea nd critical reviewo f the manuscripta, nd Yvonne Guptill for excellents ecretariahl elp. This work was supportedb y USPHS researchg rant AI 15267,t raining grant CA 09138( CHC) and a grant from the MinnesotaM edical Foundation.
- C58 mice
- in situ hybridization
- lactate dehydrogenase elevating virus