Cost-efficient entrapment of β-glucosidase in nanoscale latex and silicone polymeric thin films for use as stable biocatalysts

Muhammad Rizwan Javed, Andreas Buthe, Muhammad Hamid Rashid, Ping Wang

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

β-Glucosidase is an ubiquitous enzyme which has enormous biotechnological applications. Its deficiency in natural enzyme preparations is often overcome by exogenous supplementation, which further increases the enzyme utilization cost. Enzyme immobilization offers a potential solution through enzyme recycling and easy recovery. In the present work Aspergillus niger β-glucosidase is immobilized within nanoscale polymeric materials (polyurethane, latex and silicone), through entrapment, and subsequently coated onto a fibrous support. Highest apparent activity (90 U g-1 polymer) was observed with latex, while highest entrapment efficiency (93%) was observed for the silicone matrix. Immobilization resulted in the thermo-stabilization of the β-glucosidase with an increase in optimum temperature and activation energy for cellobiose hydrolysis. Supplementation to cellulases also resulted in an increased cellulose hydrolysis, while retaining more than 70% functional stability. Hence, the current study describes novel preparations of immobilized β-glucosidase as highly stable and active catalysts for industrial food- and bio-processing applications.

Original languageEnglish (US)
Pages (from-to)1078-1085
Number of pages8
JournalFood Chemistry
Volume190
DOIs
StatePublished - Jun 29 2016

Bibliographical note

Funding Information:
The Higher Education Commission ( HEC ) and Pakistan Atomic Energy Commission ( PAEC ) of Pakistan are acknowledged for providing the financial support to carry out the current research. The authors are also grateful for the contributions of members of Nanobiotechnology Lab, Department of Bioproducts and Biosystems Engineering, Biotechnology Institute, University of Minnesota, USA.

Publisher Copyright:
© 2015 Elsevier Ltd. All rights reserved.

Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.

Keywords

  • Immobilization
  • Latex
  • Polyurethane
  • Silicone
  • β-Glucosidase

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