TY - JOUR
T1 - Creation of monosomic derivatives of human cultured cell lines
AU - Clarke, Duncan J.
AU - Giménez-Abián, Juan F.
AU - Tönnies, Holger
AU - Neitzel, Heidemarie
AU - Sperling, Karl
AU - Downes, C. Stephen
AU - Johnson, Robert T.
PY - 1998/1/6
Y1 - 1998/1/6
N2 - Monosomic mammalian cell lines would be ideal for studying gene dosage effects, including gene imprinting, and for systematic isolation of recessive somatic mutants parallel to the invaluable mutants derived from haploid yeast. But autosomal monosomies are lethal in early development; although monosomies appear in tumors, deriving cell lines from these tumors is difficult and cannot provide several syngenic lines. We have developed a strategy for generating stable monosomic human cells, based on random autosomal Integration of the gpt plasmid, partial inhibition of DNA topoisomerase II during mitosis to promote chromatid non- disjunction, and selection against retention of gpt. These are likely to be valuable as a source of otherwise inaccessible mutants. The strategy can also be used to generate partial mammalian monosomies, which are desirable as a source of information on recessive genes and gene imprinting.
AB - Monosomic mammalian cell lines would be ideal for studying gene dosage effects, including gene imprinting, and for systematic isolation of recessive somatic mutants parallel to the invaluable mutants derived from haploid yeast. But autosomal monosomies are lethal in early development; although monosomies appear in tumors, deriving cell lines from these tumors is difficult and cannot provide several syngenic lines. We have developed a strategy for generating stable monosomic human cells, based on random autosomal Integration of the gpt plasmid, partial inhibition of DNA topoisomerase II during mitosis to promote chromatid non- disjunction, and selection against retention of gpt. These are likely to be valuable as a source of otherwise inaccessible mutants. The strategy can also be used to generate partial mammalian monosomies, which are desirable as a source of information on recessive genes and gene imprinting.
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U2 - 10.1073/pnas.95.1.167
DO - 10.1073/pnas.95.1.167
M3 - Article
C2 - 9419347
AN - SCOPUS:0031892735
SN - 0027-8424
VL - 95
SP - 167
EP - 171
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 1
ER -