Abstract
CRISPR-Cas-based transcriptional activators allow genetic engineers to specifically induce expression of one or many target genes in trans. Here we review the many design variations of these versatile tools and compare their effectiveness in different eukaryotic systems. Lastly, we highlight several applications of programmable transcriptional activation to interrogate and engineer complex biological processes.
Original language | English (US) |
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Pages (from-to) | 350-364 |
Number of pages | 15 |
Journal | CRISPR Journal |
Volume | 3 |
Issue number | 5 |
DOIs | |
State | Published - Oct 1 2020 |
Bibliographical note
Publisher Copyright:© Copyright 2020, Mary Ann Liebert, Inc.