CRISPR/Cas9-Mediated Genome Engineering of Primary Human B Cells

Kanut Laoharawee; Matthew J Johnson; Branden S Moriarity

doi:10.1007/978-1-0716-0290-4_24

CRISPR/Cas9-Mediated Genome Engineering of Primary Human B Cells

Kanut Laoharawee, Matthew J Johnson, Branden S Moriarity

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Abstract

The CRISPR/Cas9 system allows for site-specific gene editing and genome engineering of primary human cells. Here we describe methods for gene editing and genome engineering of B cells isolated from human peripheral blood mononuclear cells using CRISPR/Cas9. Editing frequencies of up to 90% and integration rates greater than 60% can be achieved with this method.

Original language	English (US)
Pages (from-to)	435-444
Number of pages	10
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	2115
DOIs	https://doi.org/10.1007/978-1-0716-0290-4_24
State	Published - Feb 1 2020

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title = "CRISPR/Cas9-Mediated Genome Engineering of Primary Human B Cells",

abstract = "The CRISPR/Cas9 system allows for site-specific gene editing and genome engineering of primary human cells. Here we describe methods for gene editing and genome engineering of B cells isolated from human peripheral blood mononuclear cells using CRISPR/Cas9. Editing frequencies of up to 90% and integration rates greater than 60% can be achieved with this method.",

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AU - Laoharawee, Kanut

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AU - Moriarity, Branden S

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AB - The CRISPR/Cas9 system allows for site-specific gene editing and genome engineering of primary human cells. Here we describe methods for gene editing and genome engineering of B cells isolated from human peripheral blood mononuclear cells using CRISPR/Cas9. Editing frequencies of up to 90% and integration rates greater than 60% can be achieved with this method.

U2 - 10.1007/978-1-0716-0290-4_24

DO - 10.1007/978-1-0716-0290-4_24

M3 - Article

C2 - 32006415

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JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

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CRISPR/Cas9-Mediated Genome Engineering of Primary Human B Cells

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