Abstract
2′-O or 3′-O-propargylated adenosines and ribothymidines were used as click targets for cross-linking of oligonucleotides with aliphatic and aromatic azides. The cross-link generates a sugar modification at the 2′-O-ligation site. Inexpensive ribonucleosides were used as starting materials. Cross-linking of oligonucleotides was performed at internal or terminal positions. Hybridization of homodimers with two complementary single strands resulted in stable ligated DNA duplexes.
Original language | English (US) |
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Pages (from-to) | 4460-4465 |
Number of pages | 6 |
Journal | Journal of Organic Chemistry |
Volume | 77 |
Issue number | 9 |
DOIs | |
State | Published - May 4 2012 |