Crosslinking of the T cell-specific accessory molecules CD7 and CD28 modulates T cell adhesion

Yoji Shimizu, Gijs A. Van Seventer, Elizabeth Ennis, Walter Newman, Kevin J. Horgan, Stephen Shaw

Research output: Contribution to journalArticlepeer-review

189 Scopus citations

Abstract

Regulated adhesion enables T cells to migrate through tissue and transiently interact with an endless succession of cells. Monoclonal antibody (mAb) engagement of the CD3/T cell receptor (TCR) complex results in a rapid and transient augmentation of the adhesion function of LFA-1 and VLA integrin molecules on human T cells. We show in this study that mAb crosslinking of the T cell-specific accessory molecules CD7 and CD28, or treatment with the Ca2+ ionophore A23187, results in the rapid induction of integrin-mediated adhesion to three distinct ligands: the extracellular matrix protein fibronectin, and the cell surface molecules ICAM-1 and VCAM-1. Like CD3 crosslinking, increased adhesion via CD7 and CD28 crosslinking appears to involve both protein kinase C (PKC) and cAMP-dependent protein kinases. In contrast, A23187 induction of adhesion is unaffected by PKC inhibitors. CD7 is preferentially expressed on naive T cells and is unique in being a potent inducer of naive T cell adhesion. Enhanced expression/function of adhesion-inducing molecules thus overcomes relative deficits in adhesion receptor expression.

Original languageEnglish (US)
Pages (from-to)577-582
Number of pages6
JournalJournal of Experimental Medicine
Volume175
Issue number2
DOIs
StatePublished - Feb 1 1992

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