Crystallization and preliminary X-ray diffraction analysis of the hyperthermophilic Sulfolobus solfataricus phosphotriesterase

Mikael Elias, Jérôme Dupuy, Luigia Merone, Claude Lecomte, Mosè Rossi, Patrick Masson, Giuseppe Manco, Eric Chabriere

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Organophosphates constitute the largest class of insecticides used worldwide and some of them are potent nerve agents. Consequently, organophosphate-degrading enzymes are of paramount interest as they could be used as bioscavengers and biodecontaminants. Phosphotriesterases (PTEs) are capable of hydrolyzing these toxic compounds with high efficiency. A distant and hyperthermophilic representative of the PTE family was cloned from the archeon Sulfolobus solfataricus MT4, overexpressed in Escherichia coli and crystallized; the crystals diffracted to 2.54 Å resolution. Owing to its exceptional thermostability, this PTE may be an excellent candidate for obtaining an efficient organophosphate biodecontaminant. Here, the crystallization conditions and data collection for the hyperthermophilic S. solfataricus PTE are reported.

Original languageEnglish (US)
Pages (from-to)553-555
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume63
Issue number7
DOIs
StatePublished - Jun 15 2007

Keywords

  • Organophosphate-degrading enzymes
  • Phosphotriesterases
  • Sulfolobus solfataricus

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