Culturable mycobiome of soya bean cyst nematode (Heterodera glycines) cysts from a long-term soya bean-corn rotation system is dominated by Fusarium

Deepak Haarith; Weiming Hu; Dong gyu Kim; David N. Showalter; Senyu Chen; Kathryn E. Bushley

doi:10.1016/j.funeco.2019.08.001

Culturable mycobiome of soya bean cyst nematode (Heterodera glycines) cysts from a long-term soya bean-corn rotation system is dominated by Fusarium

Deepak Haarith, Weiming Hu, Dong gyu Kim, David N. Showalter, Senyu Chen, Kathryn E. Bushley

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The cyst of the soya bean cyst nematode (SCN; Heterodera glycines), an economically important pathogen of soya beans worldwide, represents a unique microhabitat in soil. The fungi inhabiting cysts may include natural antagonists of the SCN as well as saprotrophs and other opportunists. This study aimed to characterise the entire culturable mycobiome of SCN cysts obtained from a long-term soya bean-corn rotation experiment using ITS fungal barcoding. Fusarium was consistently the most frequently isolated taxon across all sampling time points and crop sequences, followed by Ilyonectria. Among fourteen genera frequently isolated from SCN cysts, five fell within the single family Nectriaceae (Sordariomycetes) and five within the order Pleosporales (Dothideomycetes), suggesting independent evolutionary origins and shared adaptations in these groups towards colonisation of SCN cysts. Six genera (Pochonia, Clonostachys, Fusarium, Neonectria, Alternaria, and Leptosphaeria) varied significantly by crop sequence in at least one year.

Original language	English (US)
Article number	100857
Journal	Fungal Ecology
Volume	42
DOIs	https://doi.org/10.1016/j.funeco.2019.08.001
State	Published - Dec 2019

Bibliographical note

Funding Information:
This research was supported by United States Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA) grant 2015-67013-23419 and the Minnesota Soybean Growers Research and Production Council . The authors acknowledge the contributions of Dr. Yingbo Zhu in culturing fungi, technicians Mr. Wayne Gottschalk, Mrs. Cathy Johnson, Ms. Hannah Neigebauer, and Ms. Yen Vuong and summer interns at the University of Minnesota Southern Research and Outreach Station's nematology laboratory towards maintenance of the long-term rotation, and field sampling and processing. DH thanks Mr. Tae Joon Byun, a graduate student in computer science at the University of Minnesota for helping develop the dhtrim.py program. The authors also acknowledge Mr. Noah Strom, Dr. Rodrigo Olarte and the Kistler Lab for their various inputs and suggestions.

Publisher Copyright:
© 2019 The Authors

Keywords

Culturable mycobiome
Fungal community analyses
Fusarium
Heterodera glycines
Ilyonectria
Nectriaceae
SCN
Soybean cyst nematode

Access

10.1016/j.funeco.2019.08.001

OpenUrl availability

Full text

Cite this

@article{064c4852e08540fe975a1da99570a150,

title = "Culturable mycobiome of soya bean cyst nematode (Heterodera glycines) cysts from a long-term soya bean-corn rotation system is dominated by Fusarium",

abstract = "The cyst of the soya bean cyst nematode (SCN; Heterodera glycines), an economically important pathogen of soya beans worldwide, represents a unique microhabitat in soil. The fungi inhabiting cysts may include natural antagonists of the SCN as well as saprotrophs and other opportunists. This study aimed to characterise the entire culturable mycobiome of SCN cysts obtained from a long-term soya bean-corn rotation experiment using ITS fungal barcoding. Fusarium was consistently the most frequently isolated taxon across all sampling time points and crop sequences, followed by Ilyonectria. Among fourteen genera frequently isolated from SCN cysts, five fell within the single family Nectriaceae (Sordariomycetes) and five within the order Pleosporales (Dothideomycetes), suggesting independent evolutionary origins and shared adaptations in these groups towards colonisation of SCN cysts. Six genera (Pochonia, Clonostachys, Fusarium, Neonectria, Alternaria, and Leptosphaeria) varied significantly by crop sequence in at least one year.",

keywords = "Culturable mycobiome, Fungal community analyses, Fusarium, Heterodera glycines, Ilyonectria, Nectriaceae, SCN, Soybean cyst nematode",

author = "Deepak Haarith and Weiming Hu and Kim, {Dong gyu} and Showalter, {David N.} and Senyu Chen and Bushley, {Kathryn E.}",

note = "Funding Information: This research was supported by United States Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA) grant 2015-67013-23419 and the Minnesota Soybean Growers Research and Production Council . The authors acknowledge the contributions of Dr. Yingbo Zhu in culturing fungi, technicians Mr. Wayne Gottschalk, Mrs. Cathy Johnson, Ms. Hannah Neigebauer, and Ms. Yen Vuong and summer interns at the University of Minnesota Southern Research and Outreach Station's nematology laboratory towards maintenance of the long-term rotation, and field sampling and processing. DH thanks Mr. Tae Joon Byun, a graduate student in computer science at the University of Minnesota for helping develop the dhtrim.py program. The authors also acknowledge Mr. Noah Strom, Dr. Rodrigo Olarte and the Kistler Lab for their various inputs and suggestions. Publisher Copyright: {\textcopyright} 2019 The Authors",

year = "2019",

month = dec,

doi = "10.1016/j.funeco.2019.08.001",

language = "English (US)",

volume = "42",

journal = "Fungal Ecology",

issn = "1754-5048",

publisher = "Elsevier BV",

}

TY - JOUR

T1 - Culturable mycobiome of soya bean cyst nematode (Heterodera glycines) cysts from a long-term soya bean-corn rotation system is dominated by Fusarium

AU - Haarith, Deepak

AU - Hu, Weiming

AU - Kim, Dong gyu

AU - Showalter, David N.

AU - Chen, Senyu

AU - Bushley, Kathryn E.

N1 - Funding Information: This research was supported by United States Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA) grant 2015-67013-23419 and the Minnesota Soybean Growers Research and Production Council . The authors acknowledge the contributions of Dr. Yingbo Zhu in culturing fungi, technicians Mr. Wayne Gottschalk, Mrs. Cathy Johnson, Ms. Hannah Neigebauer, and Ms. Yen Vuong and summer interns at the University of Minnesota Southern Research and Outreach Station's nematology laboratory towards maintenance of the long-term rotation, and field sampling and processing. DH thanks Mr. Tae Joon Byun, a graduate student in computer science at the University of Minnesota for helping develop the dhtrim.py program. The authors also acknowledge Mr. Noah Strom, Dr. Rodrigo Olarte and the Kistler Lab for their various inputs and suggestions. Publisher Copyright: © 2019 The Authors

PY - 2019/12

Y1 - 2019/12

N2 - The cyst of the soya bean cyst nematode (SCN; Heterodera glycines), an economically important pathogen of soya beans worldwide, represents a unique microhabitat in soil. The fungi inhabiting cysts may include natural antagonists of the SCN as well as saprotrophs and other opportunists. This study aimed to characterise the entire culturable mycobiome of SCN cysts obtained from a long-term soya bean-corn rotation experiment using ITS fungal barcoding. Fusarium was consistently the most frequently isolated taxon across all sampling time points and crop sequences, followed by Ilyonectria. Among fourteen genera frequently isolated from SCN cysts, five fell within the single family Nectriaceae (Sordariomycetes) and five within the order Pleosporales (Dothideomycetes), suggesting independent evolutionary origins and shared adaptations in these groups towards colonisation of SCN cysts. Six genera (Pochonia, Clonostachys, Fusarium, Neonectria, Alternaria, and Leptosphaeria) varied significantly by crop sequence in at least one year.

AB - The cyst of the soya bean cyst nematode (SCN; Heterodera glycines), an economically important pathogen of soya beans worldwide, represents a unique microhabitat in soil. The fungi inhabiting cysts may include natural antagonists of the SCN as well as saprotrophs and other opportunists. This study aimed to characterise the entire culturable mycobiome of SCN cysts obtained from a long-term soya bean-corn rotation experiment using ITS fungal barcoding. Fusarium was consistently the most frequently isolated taxon across all sampling time points and crop sequences, followed by Ilyonectria. Among fourteen genera frequently isolated from SCN cysts, five fell within the single family Nectriaceae (Sordariomycetes) and five within the order Pleosporales (Dothideomycetes), suggesting independent evolutionary origins and shared adaptations in these groups towards colonisation of SCN cysts. Six genera (Pochonia, Clonostachys, Fusarium, Neonectria, Alternaria, and Leptosphaeria) varied significantly by crop sequence in at least one year.

KW - Culturable mycobiome

KW - Fungal community analyses

KW - Fusarium

KW - Heterodera glycines

KW - Ilyonectria

KW - Nectriaceae

KW - SCN

KW - Soybean cyst nematode

UR - http://www.scopus.com/inward/record.url?scp=85072307133&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85072307133&partnerID=8YFLogxK

U2 - 10.1016/j.funeco.2019.08.001

DO - 10.1016/j.funeco.2019.08.001

M3 - Article

AN - SCOPUS:85072307133

SN - 1754-5048

VL - 42

JO - Fungal Ecology

JF - Fungal Ecology

M1 - 100857

ER -

Culturable mycobiome of soya bean cyst nematode (Heterodera glycines) cysts from a long-term soya bean-corn rotation system is dominated by Fusarium

Abstract

Bibliographical note

Keywords

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this