Cutting edge: In vivo identification of TCR redistribution and polarized IL-2 production by naive CD4 T cells

P. Reichert, R. Lee Reinhardt, E. Ingulli, M. K. Jenkins

Research output: Contribution to journalArticlepeer-review

65 Scopus citations

Abstract

TCR aggregation at the point of contact with an APC is thought to play an important role in T cell signal transduction. However, this potentially important phenomenon has never been documented during an immune response in vivo. Here we used immunohistology to show that the TCR on naive Agspecific CD4 T cells in the lymph nodes of mice injected with Ag redistributed to one side of the cell. In cases where the APC could be identified, the TCR was concentrated on the side of the T cell facing the APC. In those T cells that produced IL-2, the TCR and IL-2 localized to the same side of the cell. In vivo IL-2 production depended on costimulatory signaling through CD28, whereas TCR redistribution did not. These results show that Ag-stimulated CD4 T cells produce IL-2 in a polarized fashion and undergo CD28-independent TCR redistribution in vivo.

Original languageEnglish (US)
Pages (from-to)4278-4281
Number of pages4
JournalJournal of Immunology
Volume166
Issue number7
DOIs
StatePublished - Apr 1 2001

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