Cutting edge: Intravascular staining redefines lung CD8 T cell responses

Kristin G. Anderson, Heungsup Sung, Cara N. Skon, Leo Lefrancois, Angela Deisinger, Vaiva Vezys, David Masopust

Research output: Contribution to journalArticlepeer-review

147 Scopus citations

Abstract

Nonlymphoid T cell populations control local infections and contribute to inflammatory diseases, thus driving efforts to understand the regulation of their migration, differentiation, and maintenance. Numerous observations indicate that T cell trafficking and differentiation within the lung are starkly different from what has been described in most nonlymphoid tissues, including intestine and skin. After systemic infection, we found that >95% of memory CD8 T cells isolated from mouse lung via standard methods were actually confined to the pulmonary vasculature, despite perfusion. A respiratory route of challenge increased virus-specific T cell localization within lung tissue, although only transiently. Removing blood-borne cells from analysis by the simple technique of intravascular staining revealed distinct phenotypic signatures and chemokine-dependent trafficking restricted to Ag-experienced T cells. These results precipitate a revised model for pulmonary T cell trafficking and differentiation and a re-evaluation of studies examining the contributions of pulmonary T cells to protection and disease.

Original languageEnglish (US)
Pages (from-to)2702-2706
Number of pages5
JournalJournal of Immunology
Volume189
Issue number6
DOIs
StatePublished - Sep 15 2012

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