TY - JOUR
T1 - Cutting edge
T2 - Intravascular staining redefines lung CD8 T cell responses
AU - Anderson, Kristin G.
AU - Sung, Heungsup
AU - Skon, Cara N.
AU - Lefrancois, Leo
AU - Deisinger, Angela
AU - Vezys, Vaiva
AU - Masopust, David
PY - 2012/9/15
Y1 - 2012/9/15
N2 - Nonlymphoid T cell populations control local infections and contribute to inflammatory diseases, thus driving efforts to understand the regulation of their migration, differentiation, and maintenance. Numerous observations indicate that T cell trafficking and differentiation within the lung are starkly different from what has been described in most nonlymphoid tissues, including intestine and skin. After systemic infection, we found that >95% of memory CD8 T cells isolated from mouse lung via standard methods were actually confined to the pulmonary vasculature, despite perfusion. A respiratory route of challenge increased virus-specific T cell localization within lung tissue, although only transiently. Removing blood-borne cells from analysis by the simple technique of intravascular staining revealed distinct phenotypic signatures and chemokine-dependent trafficking restricted to Ag-experienced T cells. These results precipitate a revised model for pulmonary T cell trafficking and differentiation and a re-evaluation of studies examining the contributions of pulmonary T cells to protection and disease.
AB - Nonlymphoid T cell populations control local infections and contribute to inflammatory diseases, thus driving efforts to understand the regulation of their migration, differentiation, and maintenance. Numerous observations indicate that T cell trafficking and differentiation within the lung are starkly different from what has been described in most nonlymphoid tissues, including intestine and skin. After systemic infection, we found that >95% of memory CD8 T cells isolated from mouse lung via standard methods were actually confined to the pulmonary vasculature, despite perfusion. A respiratory route of challenge increased virus-specific T cell localization within lung tissue, although only transiently. Removing blood-borne cells from analysis by the simple technique of intravascular staining revealed distinct phenotypic signatures and chemokine-dependent trafficking restricted to Ag-experienced T cells. These results precipitate a revised model for pulmonary T cell trafficking and differentiation and a re-evaluation of studies examining the contributions of pulmonary T cells to protection and disease.
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U2 - 10.4049/jimmunol.1201682
DO - 10.4049/jimmunol.1201682
M3 - Article
C2 - 22896631
AN - SCOPUS:84866156557
SN - 0022-1767
VL - 189
SP - 2702
EP - 2706
JO - Journal of Immunology
JF - Journal of Immunology
IS - 6
ER -