TY - JOUR
T1 - Cutting edge
T2 - Krüppel-like factor 2 is required for phenotypic maintenance but not development of B1 B cells
AU - Hart, Geoffrey T.
AU - Peery, Stephen L.
AU - Hamilton, Sara E.
AU - Jameson, Stephen C.
PY - 2012/10/1
Y1 - 2012/10/1
N2 - Several recent studies reported that Krüppel-like factor (KLF)2 controls trafficking, development, and function of B cells. Conditional B cell KLF2 knockout mice have increased numbers of marginal zone B cells and decreased numbers of B1 phenoytpe cells. However, it was unclear whether KLF2 is required for B1 B cell development, survival, or phenotypic maintenance. We show that B1 phenotype B cells are present in neonatal mice with B cell-specific KLF2 deficiency, suggesting that B1 differentiation can occur even in the absence of KLF2. Furthermore, by use of an inducible knockout strategy, we show that deletion of KLF2 in mature B1 cells causes loss of phenotypic markers associated with B1 cell identity, but it has a minimal effect on shortterm cell survival. Taken together, our findings suggest that KLF2 is necessary for the maintenance of B1 cell identity rather than differentiation or survival of the population.
AB - Several recent studies reported that Krüppel-like factor (KLF)2 controls trafficking, development, and function of B cells. Conditional B cell KLF2 knockout mice have increased numbers of marginal zone B cells and decreased numbers of B1 phenoytpe cells. However, it was unclear whether KLF2 is required for B1 B cell development, survival, or phenotypic maintenance. We show that B1 phenotype B cells are present in neonatal mice with B cell-specific KLF2 deficiency, suggesting that B1 differentiation can occur even in the absence of KLF2. Furthermore, by use of an inducible knockout strategy, we show that deletion of KLF2 in mature B1 cells causes loss of phenotypic markers associated with B1 cell identity, but it has a minimal effect on shortterm cell survival. Taken together, our findings suggest that KLF2 is necessary for the maintenance of B1 cell identity rather than differentiation or survival of the population.
UR - http://www.scopus.com/inward/record.url?scp=84866554187&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84866554187&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.1201439
DO - 10.4049/jimmunol.1201439
M3 - Article
C2 - 22942434
AN - SCOPUS:84866554187
SN - 0022-1767
VL - 189
SP - 3293
EP - 3297
JO - Journal of Immunology
JF - Journal of Immunology
IS - 7
ER -