TY - JOUR
T1 - Cutting edge
T2 - MicroRNA-181 promotes human NK cell development by regulating notch signaling
AU - Cichocki, Frank M
AU - Felices, Martin
AU - Mc Cullar, Valarie J
AU - Presnell, Steven R.
AU - Al-Attar, Ahmad
AU - Lutz, Charles T.
AU - Miller, Jeffrey S
PY - 2011/12/15
Y1 - 2011/12/15
N2 - MicroRNAs (miRs) have recently been identified as important regulators of gene expression at the posttranscriptional level. Although it has clearly been established that miRs influence the ontogeny of several immune cell lineages, the role of individual miRs during NK cell development has not been described. In this study, we show that miR-181 expression levels have a profound impact on the development of human NK cells from CD34 + hematopoietic progenitor cells and IFN-γ production in primary CD56 + NK cells. We also demonstrate that nemo-like kinase (NLK), an inhibitor of Notch signaling, is a target of miR-181 in NK cells, and knockdown of NLK mirrors the developmental effect of miR-181 overexpression. We conclude that miR-181 promotes NK cell development, at least in part, through the suppression of NLK, providing an important link between miRs and Notch signaling.
AB - MicroRNAs (miRs) have recently been identified as important regulators of gene expression at the posttranscriptional level. Although it has clearly been established that miRs influence the ontogeny of several immune cell lineages, the role of individual miRs during NK cell development has not been described. In this study, we show that miR-181 expression levels have a profound impact on the development of human NK cells from CD34 + hematopoietic progenitor cells and IFN-γ production in primary CD56 + NK cells. We also demonstrate that nemo-like kinase (NLK), an inhibitor of Notch signaling, is a target of miR-181 in NK cells, and knockdown of NLK mirrors the developmental effect of miR-181 overexpression. We conclude that miR-181 promotes NK cell development, at least in part, through the suppression of NLK, providing an important link between miRs and Notch signaling.
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UR - http://www.scopus.com/inward/citedby.url?scp=83755173483&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.1100835
DO - 10.4049/jimmunol.1100835
M3 - Article
C2 - 22084432
AN - SCOPUS:83755173483
SN - 0022-1767
VL - 187
SP - 6171
EP - 6175
JO - Journal of Immunology
JF - Journal of Immunology
IS - 12
ER -