TY - JOUR
T1 - Cytological analysis of chromosome structural defects that result from topoisomerase II dysfunction.
AU - Giménez-Abián, Juan F.
AU - Clarke, Duncan J.
PY - 2009
Y1 - 2009
N2 - For analyzing chromosome structural defects that result from topoisomerase II (topo II) dysfunction, we have adapted classical cell cycle experiments, classical cytological techniques, and the use of a potent topo II inhibitor (ICRF-193). In this chapter, we describe in detail the protocols used and we discuss the rationale for our choice and for the adaptations applied. We clarify in which cell cycle stages each of the different chromosomal aberrations induced by inhibiting topo II take place: lack of chromosome segregation, undercondensation, lack of sister chromatid resolution, and lack of chromosome individualization. We also put these observations into the context of the two topo II-dependent cell cycle checkpoints.
AB - For analyzing chromosome structural defects that result from topoisomerase II (topo II) dysfunction, we have adapted classical cell cycle experiments, classical cytological techniques, and the use of a potent topo II inhibitor (ICRF-193). In this chapter, we describe in detail the protocols used and we discuss the rationale for our choice and for the adaptations applied. We clarify in which cell cycle stages each of the different chromosomal aberrations induced by inhibiting topo II take place: lack of chromosome segregation, undercondensation, lack of sister chromatid resolution, and lack of chromosome individualization. We also put these observations into the context of the two topo II-dependent cell cycle checkpoints.
UR - http://www.scopus.com/inward/record.url?scp=73949112214&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=73949112214&partnerID=8YFLogxK
U2 - 10.1007/978-1-60761-340-4_15
DO - 10.1007/978-1-60761-340-4_15
M3 - Article
C2 - 19763951
AN - SCOPUS:73949112214
SN - 1064-3745
VL - 582
SP - 189
EP - 207
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -