Survival of human bronchial epithelial cells after administration of four chemical carcinogens was measured in a donal assay. Human bronchial epithelial cells were obtained from outgrowths of explanted tissue pieces. Serum-free medium was used for both explant culture and donal growth. The donal assay could be performed on three substrata: plastic dishes alone, protein-coated dishes, and inactivated Swiss 3T3 cells. Several other cell lines supported donal growth of the human cells. Fetal bovine serum was inhibitory to colony formation on plastic and protein-coated dishes, but had no effect on the growth of bronchial cells on 3T3 feeder cells. Little variation among individuals in cytotoxicity of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and K2CrO4, a possible human lung carcinogen, was observed, but in the case of benzo[a]pyrene (BP) and 7,12-dimethylbenz[a]anthracene (DMBA), large variations in survival were found between cultures derived from different individuals.
Bibliographical noteFunding Information:
The authors thank Dr George Michalopoulos and Mr Alan Novotny for providing human tissue, and Mrs Linda Scearce for manuscript preparation. The technical assistance of Ms Mary Smith and Mrs Deborah Hanna Pollard is gratefully acknowledged. Preliminary accounts of this work have been presented (1). This work was supported by the U.S. Environmental Protection Agency contract 68-02-4031. This report has been reviewed by the Health Effects Research Laboratory, U.S. Environmental Protection Agency, and approved for publication. Approval does not signify that the contents reflect the views and policies of the U.S. Environmental Protection Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use.