An important tool to study the regulation of gene expression is the sequencing and the analysis of different RNA fractions: total, ribosome-free, monosomal and polysomal. By comparing these different populations, it is possible to identity which genes are differentially expressed and to get information on how transcriptional and translational regulation modulates cellular function. Therefore, we used this strategy to analyze the regulation of gene expression of human adipose-derived stem cells during the triggering of the adipogenic and osteogenic differentiation. Here, we have focused on analyzing the differential expression of mRNAs during early adipogenic and osteogenic differentiation, and presented the detailed data concerning the experimental design, the RNA-Seq quality data, the raw data obtained and the RT-qPCR validation data. This information is important to confirm the accuracy of the data considering a future reuse of the data provided. Moreover, this study may be used as groundwork for future characterization of the transcriptome and the translatome regulation of different cell types.
Bibliographical noteFunding Information:
The authors thank the Program for Technological Development in Tools for Health-PDTIS FIOCRUZ for allowing them to use their facilities. This work was supported by grants from Fundação Araucária , FIOCRUZ , CAPES and CNPq . We thank Wagner Nagib de Souza Birbeire for the graphic design help.
© 2019 The Author(s)
- Human adipose-derived stem cells
- Polysome profiling
PubMed: MeSH publication types
- Journal Article