De novo characterization of genes that contribute to high-level ciprofloxacin resistance in Escherichia coli

Thu Tran, Qinghong Ran, Lev Ostrer, Arkady Khodursky

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Sensitization of resistant bacteria to existing antibiotics depends on the identification of candidate targets whose activities contribute to resistance. Using a transposon insertion library in an Escherichia coli mutant that was 2,000 times less susceptible to ciprofloxacin than its parent and the relative fitness scores, we identified 19 genes that contributed to the acquired ciprofloxacin resistance and mapped the shortest genetic path that increased the antibiotic susceptibility of the resistant bacteria back to a near wild-type level.

Original languageEnglish (US)
Pages (from-to)6353-6355
Number of pages3
JournalAntimicrobial agents and chemotherapy
Volume60
Issue number10
DOIs
StatePublished - Oct 2016

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