Cryostat sections of 11 rejected human renal grafts were selected for positive results in immunofluorescence tests. Immune complexes were detected in glomerular basement membrane (9 cases), tubular basement membrane (3 cases), and vessels (5 cases). Preincubation of the sections with FII of pooled human serum, but not of rabbit serum, prevented the staining of immune complexes for human IgG. These findings were interpreted by the assumption that most complexes under study were produced by reaction of the patient's altered IgG with the rheumatoid-like factor of IgG variety.
Bibliographical noteFunding Information:
This work was supported by NIH Research Grant AM-17317.