TY - JOUR
T1 - Degradation of high concentration 2,4-dichlorophenol by simultaneous photocatalytic-enzymatic process using TiO 2/UV and laccase
AU - Jia, Jingbo
AU - Zhang, Songping
AU - Wang, Ping
AU - Wang, Huajun
PY - 2012/2/29
Y1 - 2012/2/29
N2 - Removal of 2,4-dichlorophenol (2,4-DCP) by TiO 2/UV photocatalytic, laccase, and simultaneous photocatalytic-enzymatic treatments were investigated. Coupling of native laccase with TiO 2/UV showed a negative synergetic effect due to the rapid inactivation of laccase. Immobilizing laccase covalently to controlled porous glass (CPG) effectively enhanced the stability of laccase against TiO 2/UV induced inactivation. By coupling CPG-laccase with the TiO 2/UV the degradation efficiency of 2,4-DCP was significantly increased as compared with the results obtained when immobilized laccase or TiO 2/UV were separately used. Moreover, the enhancement was more remarkable for the degradation of 2,4-DCP with high concentration, such that for the degradation of 5mM 2,4-DCP, 90% removal percentage was achieved within 2h with the coupled degradation process. While for the TiO 2/UV and CPG-laccase process, the removal percentage of 2,4-DCP at 2h were only 26.5% and 78.1%, respectively. The degradation kinetics were analyzed using a intermediate model by taking into account of the intermediates formed during the degradation of 2,4-DCP. The high efficiency of the coupled degradation process therefore provided a novel strategy for degradation of concentrated 2,4-DCP. Furthermore, a thermometric biosensor using the immobilized laccase as biorecognition element was constructed for monitoring the degradation of 2,4-DCP, the result indicated that the biosensor was precise and sensitive.
AB - Removal of 2,4-dichlorophenol (2,4-DCP) by TiO 2/UV photocatalytic, laccase, and simultaneous photocatalytic-enzymatic treatments were investigated. Coupling of native laccase with TiO 2/UV showed a negative synergetic effect due to the rapid inactivation of laccase. Immobilizing laccase covalently to controlled porous glass (CPG) effectively enhanced the stability of laccase against TiO 2/UV induced inactivation. By coupling CPG-laccase with the TiO 2/UV the degradation efficiency of 2,4-DCP was significantly increased as compared with the results obtained when immobilized laccase or TiO 2/UV were separately used. Moreover, the enhancement was more remarkable for the degradation of 2,4-DCP with high concentration, such that for the degradation of 5mM 2,4-DCP, 90% removal percentage was achieved within 2h with the coupled degradation process. While for the TiO 2/UV and CPG-laccase process, the removal percentage of 2,4-DCP at 2h were only 26.5% and 78.1%, respectively. The degradation kinetics were analyzed using a intermediate model by taking into account of the intermediates formed during the degradation of 2,4-DCP. The high efficiency of the coupled degradation process therefore provided a novel strategy for degradation of concentrated 2,4-DCP. Furthermore, a thermometric biosensor using the immobilized laccase as biorecognition element was constructed for monitoring the degradation of 2,4-DCP, the result indicated that the biosensor was precise and sensitive.
KW - 2,4-Dichlorophenol
KW - Degradation
KW - Laccase
KW - Photocatalysis
KW - TiO
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UR - http://www.scopus.com/inward/citedby.url?scp=84856214397&partnerID=8YFLogxK
U2 - 10.1016/j.jhazmat.2011.12.052
DO - 10.1016/j.jhazmat.2011.12.052
M3 - Article
C2 - 22236949
AN - SCOPUS:84856214397
VL - 205-206
SP - 150
EP - 155
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
SN - 0304-3894
ER -