A cell free extract of an indolmycin-producing strain of Streptomycesgriseus is shown to catalyze the transfer of a methyl group from S-adenosylmethionine-(methyl-14C) to indolepyruvate to form radioactive β-methylindolepyruvate, an intermediate in indolmycin biosynthesis. The reaction product β-methylindolepyruvate has been unequivocally identified by chemical correlations and it has been shown to have the S configuration.
|Original language||English (US)|
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - May 22 1970|
Bibliographical noteFunding Information:
We thank Chas. Pfizer and Co. for providing us with an indolmycin-producing strain of 2. griseus and with a sample of indolmycin. We are also indebted Dr. H. R. Synder, University of Illinois, for a gift of B-methyltryptophan A and B. This work was supported by U.S. Public Health Service Grant No.