A host of proteins was seen when the chloroform-methanol insoluble protein (CMIP) fraction of bovine brain myelin was transferred from polyacrylamide gels to cellulose nitrate sheets. Inoculation of rabbits with the CMIP fraction generated a number of antibodies which were demonstrated by the immunoblot technique. These antisera against CMIP contained antibodies which induced demyelination and inhibited myelin formation in central nervous system cultures. The demyelinating factor was specific for centrally myelinated fibers, and did not demyelinate peripherally myelinated axons.
Bibliographical noteFunding Information:
Supported by the Veterans Administration (F.J.S.), NIH Grants NS 13464 and NS 19411 (H.C.A.); National Multiple Sclerosis Society Grant RG 1540A1 (H.B.C.) and NIH Training Grant 2 T 32 NS-07027-05 (M.M.G.). We thank G. Hoffman, P. Kingsley, J. Langendoerfer, P. Teal and R. Wilson for technical assistance and D. Agrawal for photography.
- demyelinating antibodies
- immunobliot technique
- myelin proteins
- myelination-inhibiting antibodies
- nerve tissue cultures