TY - JOUR
T1 - Density-dependent hyperpolarization in cultured aortic smooth muscle cells
AU - Blennerhassett, M. G.
AU - Kannan, M. S.
AU - Garfield, R. E.
N1 - Copyright:
Copyright 2004 Elsevier B.V., All rights reserved.
PY - 1989
Y1 - 1989
N2 - The membrane potential (E(m)) of cultured aortic smooth muscle cells from Sprague-Dawley (SD), Wistar-Kyoto (WKY), and spontaneously hypertensive (SHR) rats was measured in proliferating primary cultures. E(m) of SD cells in high-density cultures was -51 to -58 mV, whereas that of low-density cultures (1-2 days) was -30 mV. This difference was due to a continuous process of hyperpolarization during proliferation in culture. E(m) of WKY and SHR hyperpolarized similarly, from -12 to -42 and -38 mV, respectively. Hyperpolarization of E(m) of SD, WKY, and SHR cells was related to cell density rather than time in culture. E(m) may be a sensitive and significant indicator of the changes in the differentiated state expressed by proliferating smooth muscle in vitro.
AB - The membrane potential (E(m)) of cultured aortic smooth muscle cells from Sprague-Dawley (SD), Wistar-Kyoto (WKY), and spontaneously hypertensive (SHR) rats was measured in proliferating primary cultures. E(m) of SD cells in high-density cultures was -51 to -58 mV, whereas that of low-density cultures (1-2 days) was -30 mV. This difference was due to a continuous process of hyperpolarization during proliferation in culture. E(m) of WKY and SHR hyperpolarized similarly, from -12 to -42 and -38 mV, respectively. Hyperpolarization of E(m) of SD, WKY, and SHR cells was related to cell density rather than time in culture. E(m) may be a sensitive and significant indicator of the changes in the differentiated state expressed by proliferating smooth muscle in vitro.
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M3 - Article
C2 - 2923198
AN - SCOPUS:0024565993
SN - 0002-9513
VL - 256
SP - 25/3
JO - American Journal of Physiology - Cell Physiology
JF - American Journal of Physiology - Cell Physiology
IS - 3
ER -