Design and Experiments with a Robot-Driven Underwater Holographic Microscope for Low-Cost In Situ Particle Measurements

Kevin Mallery, Dario Canelon, Jiarong Hong, Nikolaos Papanikolopoulos

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Microscopic analysis of microparticles in situ in diverse water environments is necessary for monitoring water quality and localizing contamination sources. Conventional sensors such as optical microscopes and fluorometers often require complex sample preparation, are restricted to small sample volumes, and are unable to simultaneously capture all pertinent details of a sample such as particle size, shape, concentration, and three-dimensional motion. This paper proposes a novel and cost-effective robotic system for mobile microscopic analysis of particles in situ at various depths which are fully controlled by the robot system itself. A miniature underwater digital in-line holographic microscope (DIHM) performs high-resolution imaging of microparticles (e.g., algae cells, plastic debris, sediments) while movement allows measurement of particle distributions covering a large area of water.

Original languageEnglish (US)
Article number32
JournalJournal of Intelligent and Robotic Systems: Theory and Applications
Volume102
Issue number2
DOIs
StatePublished - Jun 2021
Externally publishedYes

Bibliographical note

Funding Information:
This work is supported by the National Science Foundation through grants #IIS-1427014, #CNS-1439728, #CNS-1531330, #CNS-1544887, and #CNS-1939033. United States Department of Agriculture/ National Institute of Food and Agriculture has also supported this work through grant 2020-67021-30755.

Funding Information:
The authors would like to thank Jiaqi You, Anne Wilkinson, and Miki Hondzo for their knowledge of algae and assistance during deployments and David Brajkovic for work developing miniaturized holographic sensors. This work is supported by the National Science Foundation through grants #IIS-1427014, #CNS-1439728, #CNS-1531330, #CNS-1544887, and #CNS-1939033. USDA/NIFA has also supported this work through grant 2020-67021-30755.

Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer Nature B.V.

Keywords

  • Environmental monitoring
  • Miniature robot-based holographic microscope
  • Robot-based sensing for aquatic environments

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