TY - GEN
T1 - Design, construction, and performance of a carotenoid over-producing E. coli
AU - Unrean, Pornkamol
AU - Trinh, Cong
AU - Srienc, Friedrich
AU - Schmidt-Dannert, Claudia
PY - 2007/12/1
Y1 - 2007/12/1
N2 - An inverse metabolic engineering approach has been applied for an improved carotenoid production in Escherichia coli. Elementary Mode Analysis, a stoichiometry metabolic model, was used for a rational design of a carotenoid over-producing strain by identifying target genes knockout for elimination of inefficient carotenoid biosynthesis pathway. Therefore, on the basis of the model, the designed strain would produce carotenoid product in an efficient manner. To validate the model, we have constructed the designed strain CRT028 and tested its performance on carotenoid synthesis. This multiple-genes knockout strain has experimentally revealed up to 5 times improvement on carotenoid product yield as compared with the wildtype strain under identical growth condition. The consistency between modeling and experimental result has proven Elementary Mode Analysis as a tool for guiding the rational direction in the cell's metabolic engineer.
AB - An inverse metabolic engineering approach has been applied for an improved carotenoid production in Escherichia coli. Elementary Mode Analysis, a stoichiometry metabolic model, was used for a rational design of a carotenoid over-producing strain by identifying target genes knockout for elimination of inefficient carotenoid biosynthesis pathway. Therefore, on the basis of the model, the designed strain would produce carotenoid product in an efficient manner. To validate the model, we have constructed the designed strain CRT028 and tested its performance on carotenoid synthesis. This multiple-genes knockout strain has experimentally revealed up to 5 times improvement on carotenoid product yield as compared with the wildtype strain under identical growth condition. The consistency between modeling and experimental result has proven Elementary Mode Analysis as a tool for guiding the rational direction in the cell's metabolic engineer.
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M3 - Conference contribution
AN - SCOPUS:80053655535
SN - 9780816910229
T3 - AIChE Annual Meeting, Conference Proceedings
BT - 2007 AIChE Annual Meeting
T2 - 2007 AIChE Annual Meeting
Y2 - 4 November 2007 through 9 November 2007
ER -