TY - JOUR
T1 - Design of a phage-insensitive lactococcal dairy starter via sequential transfer of naturally occurring conjugative plasmids
AU - O'Sullivan, David
AU - Coffey, Aidan
AU - Fitzgerald, Gerald F.
AU - Hill, Colin
AU - Ross, R. Paul
PY - 1998/11
Y1 - 1998/11
N2 - The plasmid-free Lactococcus lactis subsp. cremoris MG1614 is highly phage sensitive and lacks lactose fermenting ability (Lac) and primary casein degrading ability (Prt). Food grade gene transfer systems were used to sequentially superimpose different phage defense systems on this background, resulting in a gradual increase in resistance to bacteriophage in the derivatives. pLP712, encoding Lac and Prt, was then transferred to one of these hosts, into which plasmids encoding adsorption inhibition, restriction modification, and abortive infection had already been introduced. This resulted in a phage-resistant strain which was successfully used as a single- strain starter for cheddar cheese manufacture under industrial conditions.
AB - The plasmid-free Lactococcus lactis subsp. cremoris MG1614 is highly phage sensitive and lacks lactose fermenting ability (Lac) and primary casein degrading ability (Prt). Food grade gene transfer systems were used to sequentially superimpose different phage defense systems on this background, resulting in a gradual increase in resistance to bacteriophage in the derivatives. pLP712, encoding Lac and Prt, was then transferred to one of these hosts, into which plasmids encoding adsorption inhibition, restriction modification, and abortive infection had already been introduced. This resulted in a phage-resistant strain which was successfully used as a single- strain starter for cheddar cheese manufacture under industrial conditions.
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U2 - 10.1128/aem.64.11.4618-4622.1998
DO - 10.1128/aem.64.11.4618-4622.1998
M3 - Article
AN - SCOPUS:0031764273
SN - 0099-2240
VL - 64
SP - 4618
EP - 4622
JO - Applied and environmental microbiology
JF - Applied and environmental microbiology
IS - 11
ER -