Design of a ¹³c {¹h} rf probe for monitoring the in vivo metabolism of [1‐¹³c] glucose in primate brain

The design of an RF probe suitable for obtaining proton‐decoupled ¹³C spectra from a subhuman primate brain is described. Two orthogonal saddle coils, one tuned to the resonant frequency of ¹³C and the other to the resonant frequency of ¹H, were used to monitor the in vivo metabolism of [1‐¹³C] glucose in rhesus monkey brain at 2.1 T. Difference spectra showed the appearance of ¹³C‐enriched glutamate and glutamine 30 to 40 min after a bolus injection of [ 1‐ ¹³C] glucose.