Designed signaling aptamers that transduce molecular recognition to changes in fluorescence intensity

Sulay D. Jhaveri, Romy Kirby, Rick Conrad, Emily J. Maglott, Michael Bowser, Robert T. Kennedy, Gary Glick, Andrew D. Ellington

Research output: Contribution to journalArticlepeer-review

226 Scopus citations

Abstract

We have engineered aptamers that contain fluorescent reporters and that signal the presence of cognate ligands in solution. Two different anti- adenosine 'signaling aptamers', one made from RNA and one from DNA, can selectively signal the presence of adenosine in solution. Increases in fluorescence intensity reproducibly follow increases in adenosine concentration, and can be used for quantitation. The facile methods we have developed can potentially be used for generating a wide variety of signaling aptamers for use in sensor arrays.

Original languageEnglish (US)
Pages (from-to)2469-2473
Number of pages5
JournalJournal of the American Chemical Society
Volume122
Issue number11
DOIs
StatePublished - Mar 22 2000

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