Detailed method for intrathecal delivery of gene therapeutics by direct lumbar puncture in mice

Kelsey R. Pflepsen; Cristina D. Peterson; Kelley F. Kitto; Lucy Vulchanova; George L. Wilcox; Carolyn A. Fairbanks

doi:10.1007/978-1-4939-9065-8_20

Detailed method for intrathecal delivery of gene therapeutics by direct lumbar puncture in mice

Kelsey R. Pflepsen, Cristina D. Peterson, Kelley F. Kitto, Lucy Vulchanova, George L. Wilcox, Carolyn A. Fairbanks

Research output: Chapter in Book/Report/Conference proceeding › Chapter

8 Scopus citations

Abstract

Delivery of viral vectors directly into the central nervous system (CNS) has emerged as an important tool for the refinement of gene therapy. Intrathecal delivery by direct lumbar puncture in conscious rodents offers a minimally invasive approach that avoids tissue damage and/or destruction. Here we describe delivery of small quantities of viral vector product to the intrathecal space of rodents via direct lumbar puncture aided by a catheter.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	305-312
Number of pages	8
DOIs	https://doi.org/10.1007/978-1-4939-9065-8_20
State	Published - 2019

Publication series

Name	Methods in Molecular Biology
Volume	1937
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2019.

Keywords

AAV gene therapy
Direct lumbar puncture
Gene therapy
Intrathecal
Intrathecal gene delivery

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abstract = "Delivery of viral vectors directly into the central nervous system (CNS) has emerged as an important tool for the refinement of gene therapy. Intrathecal delivery by direct lumbar puncture in conscious rodents offers a minimally invasive approach that avoids tissue damage and/or destruction. Here we describe delivery of small quantities of viral vector product to the intrathecal space of rodents via direct lumbar puncture aided by a catheter.",

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AU - Vulchanova, Lucy

AU - Wilcox, George L.

AU - Fairbanks, Carolyn A.

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KW - Intrathecal

KW - Intrathecal gene delivery

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Detailed method for intrathecal delivery of gene therapeutics by direct lumbar puncture in mice

Abstract

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Keywords

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