Detection and serotyping of dengue viruses by PCR: a simple, rapid method for the isolation of viral RNA from infected mosquito larvae.

S. Y. Chan, I. Kautner, S. K. Lam

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Dengue viruses pose a considerable global public health problem with an estimated 100 million cases of illness every year. This illustrates the need for rapid and reliable diagnostic methods for proper patient management and disease control. Currently, laboratory diagnosis depends on serology or virus isolation, with both methods having certain drawbacks. Alternatively, reverse transcription and polymerase chain reaction (RT-PCR) offers the potential for the rapid, highly sensitive and specific detection of dengue viruses. Since we occasionally encounter the problem of insufficient amounts of patient serum for the direct detection of dengue viruses, a method was developed for the extraction of viral RNA after biological amplification in mosquito larvae. Using this method, 15 of 19 clinical samples tested were correctly identified using RT-PCR.

Original languageEnglish (US)
Pages (from-to)258-261
Number of pages4
JournalThe Southeast Asian journal of tropical medicine and public health
Volume25
Issue number2
StatePublished - Jun 1 1994
Externally publishedYes

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